双黄连注射液和双黄连冻干粉针剂对家兔离体红细胞溶血作用影响.docVIP

双黄连注射液和双黄连冻干粉针剂对家兔离体红细胞溶血作用影响 　　[摘要]目的 观察双黄连注射液和双黄连冻干粉针剂对家兔离体红细胞溶血作用的影响。方法 健康家兔心脏取血20 ml，制备2%红细胞混悬液，分别与双黄连注射液、双黄连冻干粉针剂、0.9%氯化钠注射液共同温育，进行体外溶血试验，并通过分光光度计检测其吸光度，观察3 h内红细胞的溶血率。结果 浓度为0.06、0.12、0.18、0.24、0.3 g/ml的双黄连注射液的溶血率分别为8.39%、21.34%、40.98%、50.36%、67.68%，与0.9%氯化钠注射液组相比，差异有统计学意义（P0.05）。浓度为0.06、0.12、0.18、0.24、0.3 g/ml双黄连冻干粉针剂的溶血率为2.15%、5.23%、7.31%、9.59%、13.35%，在浓度为0.3 g/ml时与0.9%氯化钠注射液组相比，差异有统计学意义（P0.05）。在浓度为0.12、0.18、0.24、0.3 g/ml时，双黄连注射液与双黄连冻干粉针剂组的溶血率相比，差异有统计学意义（P0.05）。结论 双黄连粉针剂与双黄连注射液相比，对家兔离体红细胞溶血作用小。 　　[关键词]双黄连注射液；双黄连冻干粉针剂；溶血试验 　　[中图分类号] R332 [文献标识码] A [文章编号] 1674-4721（2017）04（c）-0147-03 　　[Abstract]Objective To observe the influence of Shuanghuanglian Injection and Shuanghuanglian Lyophilized Power Injection on hemolytic effect in rabbits′red blood cell （RBC） in vitro.Methods The blood samples were collected from healthy rabbits by 20 ml，and the red blood cell suspension of 2% was prepared，and it with Shuanghuanglian Injection，Shuanghuanglian Lyophilized Power Injection and Sodium Chloride Injection of 0.9% were co-incubated，and hemolysis test was carried out in vitro，and the absorbance was detected by spectrophotometer.The hemolysis rate of RBC in 3 h was observed.Results The hemolysis rate of Shuanghuanglian Injection with concentration of 0.06，0.12，0.18，0.24，0.3 g/ml was 8.39%，21.34%，40.98%，50.36%，67.68% respectively，and there was a statistical difference between two groups compared with Sodium Chloride Injection of 0.9% （P0.05）.The hemolysis rate of Shuanghuanglian Lyophilized Power Injection with concentration of 0.06，0.12，0.18，0.24，0.3 g/ml was 2.15%，5.23%，7.31%，9.59%，13.35% respectively，and there was a statistical difference when the concentration was 0.3 g/ml compared with Sodium Chloride Injection of 0.9% （P0.05）.When the concentration was 0.12，0.18，0.24，0.3 g/ml，there was a statistical difference of hemolysis rate between Shuanghuanglian Injection and Shuanghuanglian Lyophilized Power Injection （P0.05）.Conclusion The influence of Shuanghuanglian Lyop