响应面分析法优化超声提取党参皂苷工艺研究.docVIP

响应面分析法优化超声提取党参皂苷工艺研究 　　摘 要：以党参皂苷得率为衡量指标，考察了影响超声提取党参皂苷的4个因素：料液比、乙醇的质量分数、超声时间及超声功率。在单因素试验基础上，通过3因子3水平的响应面分析法进行试验，对各因子显著性和交互作用进行分析。结果表明，超声提取党参皂苷的最佳工艺条件为乙醇质量分数74.81%、超声时间39.38 min、超声功率195.10 W；该条件下党参皂苷的得率为2.589 7%。考虑到实际的操作性，以乙醇质量分数74%、超声时间39 min、超声功率200 W为条件，得到实际党参皂苷平均得率为2.564 7%，与理论值相差较小，说明该工艺条件具有实际操作性和可行性。 　　关键词：党参皂苷；响应面法；超声提取 　　中图分类号：TS202.3 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2017.10.004 　　Study on Optimization of Ultrasonic Extraction of Saponin from Codonopsis pilosula by Response Surface Method 　　WANG Jing， LIU Huiting， CHAI Qingkai， HAO Xiangrong， LI Erfeng， CHI Ming， LIU Huiqin， WANG Yuanhong 　　（College of Horticulture and Landscape Architecture， Tianjin Agricultural University， Tianjin 300384， China） 　　Abstract：In this research， the four factors of ultrasonic extraction of Codonopsis pilosula saponins were studied， including the ratio of material to liquid， the mass fraction of ethanol， ultrasonic time and ultrasonic power. On the basis of single factor experiment， the response surface analysis method of three factors and three levels was used to analyze the significance and interaction of each factor. The results showed that the optimum extraction conditions of ultrasonic extraction of Codonopsis pilosula saponins were mass fraction of ethanol 74.81%， ultrasonic time 39.38 min， ultrasonic power 195.10 W， and yield of the saponins at the optimized condition was 2.589 7%. Considering the actual operation， using the mass fraction of ethanol 74%， ultrasonic time 39 min， and ultrasonic power 200 W as the best condition， the actual average yield was 2.564 7%. The difference between the actual value and the theoretical value was very small， and it was concluded that the condition was practical and feasible. 　　Key words： saponin of Codonopsis pilosula； response surface method； ultrasonic extraction 　　党参[Codonopsis pilosula（Franch.）Nannf]属桔梗科党参属植物，别名有防风党参、狮头参、中灵草、上党参、黄参、防党参、黄党。党参是我国传统的名贵药材，开始出现于清代本草，是当山西人参资源逐渐衰败时被作为上党人参上市出现的[1]。党参提取物含有皂苷、淀粉、蔗糖、维生素B2、葡萄糖、