吸入氢气对脓毒血症大鼠急性肺损伤影响.docVIP

吸入氢气对脓毒血症大鼠急性肺损伤影响 　　[摘要] 目的 探讨吸入氢气（H2）对脓毒血症大鼠急性肺损伤的影响。 方法 选取雄性SD大鼠40只，体重200～250 g。采用随机数字表法将其分为4组（n = 10）：假手术组（SH组）、盲肠结扎穿孔组（CLP组）、1% H2组和2% H2组。采用CLP法制备脓毒血症模型。1% H2组与2% H2组于CLP术后12 h分别吸入1% H2与2% H2 2 h。结束后，采集股动脉血样行血气分析，计算氧合指数（PaO2/FiO2）；测定血浆中高迁移率族蛋白1（HMGB1）、白细胞介素-6（IL-6）水平；随后处死大鼠取肺组织，检测肺组织中Toll样受体4（TLR-4）、核因子-кB（NF-κB）、髓过氧化物酶（MPO）活性；光镜下观察肺组织病理改变。 结果 与SH组比较，CLP组、1% H2组、2% H2组PaO2/FiO2降低，血浆中HMGB1、IL-6水平升高，肺组织TLR-4、NF-κB、MPO水平升高，病理学损伤明显加重（P 0.05）。 结论 吸入H2能够减轻脓毒血症引起的急性肺损伤，其机制可能与抑制炎性反应有关。 　　[关键词] 氢气；脓毒血症；大鼠；肺损伤 　　[中图分类号] R631 [文献标识码] A [文章编号] 1673-7210（2015）09（a）-0008-03 　　[Abstract] Objective To investigate the effect of hydrogen （H2） inhalation on acute lung injury in rats with sepsis. Methods Forty healthy male SD rats （weighing 200-250 g） were randomly divided into 4 groups （n = 10） using a random number table： sham operation group （SH group）， cecal ligation and puncture group （CLP group）， 1% H2 group， 2% H2 group respectively. Sepsis model was produced by CLP. 1% H2 and 2% H2 group received 2 h inhalation of 1% H2 and 2% H2 at 12 h after CLP， respectively. At the end of hydrogen inhalation， blood samples were obtained for blood gas analysis. PaO2/FiO2 was calculated and the levels of high mobility group protein 1 （HMGB1）， interleukin-6 （IL-6） were determined. The rats were then sacrificed and lungs were immediately removed for determination of the activity of Toll-like receptors-4 （TLR-4）， nuclear factor kappa B （NF-κB） and myeloperoxidase （MPO）； pathology assessment of lung was done under light microscope. Results Compared with SH group， PaO2/FiO2 was significantly decreased， plasma levels of HMGB1， IL-6 increased， levels of TLR-4， NF-κB， MPO in lungs increased， and the pathological injury was significantly aggravated in CLP group， 1% H2 group and 2% H2 group （P 0.05）. Conclusion Hydrogen inhalation can alleviate sepsis induced ALI in rats， and its mechanism may be related to inhibiting inflammatory response. 　　[Key words] Hydrogen； Sepsis； Rat； Lung injury