地黄与白芍合煎及单煎对有效成分梓醇溶出量影响.docVIP

地黄与白芍合煎及单煎对有效成分梓醇溶出量影响 　　摘要：目的 比较地黄单煎及其与白芍合煎液中有效成分梓醇含量的变化，初步探索2味中药配伍的变化规律。方法 采用高效液相色谱法，选用Waters symmetry C18色谱柱（4.6 mm×150 mm， 5 ?m），流动相为乙腈-0.01%磷酸水等度洗脱系统，流速1.0 mL/min，检测波长210 nm，柱温25 ℃。结果 梓醇的回归方程为 Y=270 054X-57 724，r=0.999 6，在0.225～7.2 ?g范围内线性关系良好，平均加样回收率为101.15%， RSD=0.39%。地黄-白芍合煎液中梓醇含量比地黄单煎液平均升高18.66%。结论 地黄-白芍合煎液中梓醇含量比单煎液高，合煎更有助于有效成分的溶出，二者配伍具有一定的合理性。 　　关键词：地黄；白芍；梓醇；单煎；合煎；高效液相色谱法 　　DOI：10.3969/j.issn.1005-5304.2016.09.023 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2016）09-0096-03 　　Effects of Decocting Rehmanniae Radix and Paeoniae Radix Alba Single and Mixed on Dissolving-out Quantity of Catalpol CHEN Li-ping， LI Mao-xing， ZHOU Jun （Department of Pharmacy， Lanzhou General Hospital of PLA； Key Laboratory of Prevention and Cure for Plateau Environment Damage， PLA， Lanzhou 730050， China） 　　Abstract： Objective To compare the content variation of catalpol between single and mixed decocting Rehmanniae Radix and Paeoniae Radix alba； To preliminarily explore the variation rules of compatibility of Rehmanniae Radix and Paeoniae Radix alba. Methods HPLC method was used with Waters Symmetry C18 column （4.6 mm×150 mm， 5 ?m）， flow rate of 1.0 mL/min， detection wavelength of 210 nm， column temperature at 25 ℃. The mobile phase was acetonitrile-0.01% phosphoric acid in water isocratic elution system. Results Catalpol regression equation was Y=270 054X-57 724， r=0.999 6， showing good linear range among 0.225?C7.2 ?g. The average recovery rate was 101.15%， RSD=0.39%. The average content of catalpol in the mixed decoction of Rehmanniae Radix and Paeoniae Radix alba was 18.66% more than the single decoction of Rehmanniae Radix. Conclusion The content of catalpol in mixed decoction is higher than the single decoction of Rehmanniae Radix and Paeoniae Radix alba. The mixed decoction is favorable for the dissolving of catalpol from the medicine. The compatibility of Rehmanniae Radix and Paeoniae Radix alba is rational. 　　Key words： Rehmanniae Radix； Paeoniae Radix alba； catalpol； si