宁夏枸杞内生菌抗菌和抗肿瘤活性研究.docVIP

宁夏枸杞内生菌抗菌和抗肿瘤活性研究 　　[摘要] 为筛选具有抗菌抗肿瘤活性的枸杞内生菌资源，对分离自宁夏枸杞的10株内生菌发酵液的抑菌活性和体外细胞毒活性进行检测，通过ITS 和16S rDNA序列分析对菌株进行了分子鉴定。结果表明，5株内生真菌对供试病原菌都未表现出抑制活性，放线菌AL6对3种病原真菌都有一定的抑制作用，而AL5仅对金黄色葡萄球菌有较强抑制活性。但内生真菌的抗肿瘤活性明显强于放线菌。当发酵液浓度为0.2 g?L-1时，有4株内生真菌至少对1种肿瘤细胞的抑制率高于50%。序列分析表明，5株内生真菌隶属于曲霉属、青霉属和翘孢霉属。5株内生放线菌隶属于链霉菌属、微球菌属和拟诺卡氏菌属。曲霉属菌株FL1对A549和HeLa细胞的抑制活性都较强，其IC50分别为0.022，0.028 g?L-1，值得进一步研究。 　　[关键词] 枸杞；内生菌；抑菌活性；抗肿瘤活性 　　[Abstract] The antimicrobial activity and cytotoxicity in vitro of the fermentation broth of 10 endophytic strains isolated from Lycium barbarum were determined to screen high activity endophytic strains. Sequences analysis of ITS and 16S rDNA was used for molecular identification of the strains. The results showed that 5 endophytic fungi had no inhibitory activity against the tested pathogens. Endophytic actinomycete strain AL6 had a certain inhibitory effect on 3 kinds of pathogenic fungi，and strain AL5 only had strong inhibitory activity against Staphylococcus aureus. However，the anti-tumor activity of endophytic fungi was significantly higher than that of actinomycetes. Four endophytic fungi strains exhibited the growth inhibition rate of above 50% against at least one of the tested tumor cells when the concentration of fermentation broth was 0.2 g?L-1. Sequences analysis showed that 5 endophytic fungi strains belonged to genus Aspergillus，Penicillium and Emericella，and the 5 endophytic actinomycetes strains belonged to genus Aspergillus，Penicillium and Emericella. Aspergillus strain FL1 had stronger inhibitory activity against A549 and HeLa cells，and the IC50 values were 0.022，0.028 g?L-1，respectively，which was worthy of further study. 　　[Key words] Lycium barbarum；endophytes；anti-microbial activities；anti-tumor activities 　　?壬?菌（endophyte）是指一类在其部分或全部生活史中存活于健康植物组织内部，而不使宿主植物表现出明显感染症状的微生物[1]。植物内生菌在植物微生态系统中，与宿主建立了和谐的联合关系，不仅能够参与植物次生代谢及成分的转化合成，而且还能够独立产生丰富的次生代谢产物，是天然产物的重要来源[2]。药用植物内生菌次生代谢产物含有与宿主植物相同或相似的药用活性成分，包括抗肿瘤、抗菌、抗炎症、免疫调节、降糖等天然化合物[3]。直到现在人们已发现微生物的代谢产物中有60余种药理作用[4]。尤其是近1