USP4介导的TGF-β信号通路对兔耳增生性瘢痕形成的影响-外科学(整形外科)专业论文.docxVIP

Abst Abstract Abst Abstract IV IV PAGE PAGE VII ABSTRACT Hypertrophic scars are comman clinically pathological scars,and can be visible、 elevated、itchy and painful,causing serious functionl disability. Recent studies have shown that TGF-β signaling pathway plays a key role in the formation of hypertrophic scars,and other studies have found that USP4 can enhance the level of the TGF-β signaling pathway,but in the hypertrophic scars formation,there is no report about the role of USP4 for the TGF-β signaling pathway.Our previous studies show that USP4 gene significantly higher in the hypertrophic scars tissue, and was positively correlated with TβRI,futher study using USP4 inhibitors in the scar fibroblasts can significantly reduce the expression of TβRI,suggesting that USP4 may influence the formation of scar tissue through regulating the TGF-β signaling pathway. Research shows that in the process of wound repair,the rhTGF-β3 could significantly inhibit the formation of hypertrophic scars .In the experiment Ⅰ ,we applied rhTGF-β3 in the rabbit ears modle of hypertrophic scars to explore whether USP4 and TβRI in the rabbit ears hypertrophic scars high expression，and study its influence on USP4 and TβRI.Vialinin A,is an inhibitor of USP4，In the experiment Ⅱ，we applied Vialinin A to inhibit USP4,exploring the effects of interferencing USP4 on hypertrophic scars formation. We detected the expression of TβRI、TGF-β1、Smad7 in the rabbit ears scar tissue,so as to investgate the possible ways of USP4.In order to futher explore the effect on hyperplastic scar formation and explore new methods for the treatment of hyperplastic scar in animal level. This paper consists of two parts: Part Ⅰ: Study on the expression of USP4 and TβRI on the hypertrophic scars of rabbit ears Objective ： To investigate whether USP4 and TβRI in the rabbit ears hypertrophic scars high expression,study whether USP4 plays an important part in rabbit ears hypertrophic scar formati