雌二醇E2通过调控PEDF表达促进乳腺癌增殖作用探究.pdfVIP

World Journal of Cancer Research 世界肿瘤研究, 2018, 8(1), 49-54 Published Online January 2018 in Hans. /journal/wjcr /10.12677/wjcr.2018.81008 The Effect of Estradiol E2 on the Proliferation of Breast Cancer by Regulating PEDF Expression * # Honghai Hong , Weiqin Yang, Xicai Tang, Yao Feng Department of Clinical Laboratory, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou Guangdong rd th th Received: Jan. 3 , 2018; accepted: Jan. 17 , 2018; published: Jan. 24 , 2018 Abstract Objective: To investigate the molecular mechanism of estradiol E2 to promote the proliferation of breast cancer by regulating the expression PEDF. Methods: Serum PEDF level was detected by ELISA kit and estradiol E2 level was detected by radioimmunoassay; the effect of estradiol on the PEDF regulation was detected by Western blot; the proliferation of breast cancer cell line MCF-7 was detected by MTT assay. Results: The expression of PEDF was down regulated in breast cancer, and negatively correlated with estradiol E2 level. Estradiol E2 can reduce the expression of PEDF and promote the proliferation of breast cancer cell MCF-7. Conclusion: Estradiol E2 can promote the proliferation of breast cancer by regulating the expression of PEDF. Keywords Breast Cancer, Estradiol E2, Pigment Epithelial Cell Derived Factor PEDF, Proliferation 雌二醇E2通过调控PEDF表达促进乳腺癌增殖 作用研究 * # 洪宏海 ，杨卫琴，唐希才，冯 遥 广州医科大学附属第三医院检验科，广东 广州 收稿日期：2018年1月3 日；录用日期：2018年1月17 日；发布日期：2018年1月24 日 *第一作者。 #通讯作者。 文章引用: 洪宏海, 杨卫琴, 唐希才, 冯遥. 雌二醇E2 通过调控PEDF 表达促进乳腺癌增殖作用研究[J]. 世界肿瘤研究, 2018, 8(1): 49-54.