β-乳球蛋白致敏表位的适配体定位、酶解及脱敏研究-食品科学专业论文.docxVIP

万方数据 万方数据 4 Influence of hydrolysis product of β-lactoglobulinsensitization in mice Through the mice ear immediate active skin allergy test and oral sensitized mice intestinal permeability test showed that, after the beta lactoglobulin sensitized mice, compared with the blank group, Evans blue extravasation rate and intestinal index increased significantly. Aptamer QWVW, WVDL docking beta-lactoglobulin reduce sensitization effect is better. The test mice serum IgG showed that QWVW and WVDL, these two groups of aptamer should cover up the beta-lactoglobulin sensitization loci, reduced beta lactoglobulin sensitization. The aptamer QWVW corresponding to the VTQT, as well as aptamer WVDL corresponds to the TQLE beta lactoglobulin on sensitization loci. Keywords: beta-lactoglobulin; aptamer; separation and purification; sensitization III 目 录 摘 要.......................................................................................................................... I Abstract........................................................................................................................ II 文献综述........................................................................................................................3 引 言............................................................................................................................10 1 材料与方法.............................................................................................................. 11 1.1 试验材料与仪器............................................................................................ 11 1.1.2 主要仪器设备......................................................................................12 1.1.3 主要溶液配制.......................................................................................12 1.2 实验方法........................................................................................................12 1.2.1 β-乳球蛋白过敏原表位适配体的计算机辅助分子设计与筛选 12 1.2.2 中性蛋白酶与胰蛋白酶水解β-乳球蛋白条件的优化 13 1.2.3 β-乳球蛋白致