“超声增敏ADM”治疗乳腺癌的作用及机制外科学(普外)专业论文.docxVIP

华中科技大学博士学位论文 华 中 科 技 大 学 博 士 学 位 论 文 PAGE 5 PAGE 5 Effect of ultrasound-assistant ADM chemotherapy on breast cancer ABSTRACT PART ONE Detecting oxygen species arising from the activation of ADM by ultrasonic Objectives: To investigate whether ultrasound can activate ADM and produce oxygen species without change the spectral characteristics of ADM. Methods: Using ROS probe to explore the wavelength intensity of ADM, and generation of reactive oxygen species, and using fluorescence staining method to explore the level of intracellular reactive oxygen species of cells. In addition, by analyzing the absorption spectrum curve of ADM to see the change of the molecular structure of ADM. Results: There are no difference in the fluorescence spectra curve between the ultrasound before and the ultrasound after. The the intervention of ultrasound after, the ADM solution make the curve peak increased, but after the flowing test, adding SOD, and NaN3 reduced the peak; ultrasound intervention of MCF-7/ADM cells and MCF-7/S cells DCF mean fluorescence intensity were 28.4 ± 6.5, 36.7 ± 4.2 ,which were significantly lower than the corresponding ultrasound before the intervention (16.5 ± 1.8,19.3 ± 2.9) (P 0.05). Conclusions: After the above three experiments, we found that a certain wavelength intensity ultrasound can produce singlet oxygen 1O2 and the superoxide anion radical O2— (Experiment 2), and increase the level of ROS in breast cancer cells (experiment 3), without changing the molecular structure of ADM (Experiment 1). Key words: breast cancer; ADM; fluorescence spectroscopy PART TWO Detection of Ultrasound-assistant ADM chemotherapy and the reversion of drug resistance Objective: To study the effection of Ultrasound (US) assistant ADM therapy and the reversion of drug resistance Methods: A human breast cancer cells MCF-7 / S and adriamycin-resistant human breast cancer cells MCF-7/ADM were on this study. By cell growth curve, detection MCF-7/ADM resistance protein MDR1, P-