紫杉醇对胃癌细胞株cox 2表达的影响及可能的信号转导通路的研究-effects of paclitaxel on cox 2 expression and possible signal transduction pathway in gastric cancer cell line.docxVIP

PAGE PAGE 10 结论：紫杉醇可诱导 SGC-7901 COX-2 mRNA 的表达，这种表达可被 SB203580 所 抑制，并且 SB203580 可提高由紫杉醇介导的 SGC-7901 细胞的凋亡作用，P38 信号通路可能是紫杉醇诱导 SGC-7901 COX-2 表达的通路之一。 关键词：紫杉醇；环氧化酶-2；SB203580；胃癌细胞株 SGC-7901；P38 信号通 路 The effect of Paclitaxel on human gastric cancer cell lines expressed COX-2 and possible signal transduction pathway research Abstract Background ＆ Objective: COX-2 is the rate-limiting enzyme prostaglandin synthesis. Studies have shown that COX-2 expression are closely related to tumor occurrence, development and metastasis, Also affect the efficacy of chemotherapy, which may be related to tumor cells expression of COX-2 by chemotherapeutic drug induced and multidrug resistance occurrence. In vitro, the experiments have showed that chemotheraputic drug taxol can induce gastric cancer cell lines the expression of COX-2 protein and MDR protein at the same time. The expression of COX-2 can induce the expression of MDR protein, and the inhibiting COX-2 expression could reverse MDR expression. Thus, the understanding of signal pathway by paclitaxel -induced gastric cancer cell line COX-2 protein expression is of great significance for exploring the mechanism and reversing resistance in the future. In this experiment, the paclitaxel and P38 pathway signal inhibitor SB203580 were added to culturing human gastric cancer cell line SGC-7901, by comparing to the changes on COX-2 mRNA expression of SGC-7901 after paclitaxel alone and combating paclitaxel with SB203580, we can understand the role of P38 signaling pathway in the paclitaxel -induced SGC-7901 COX-2 expression. Methods: The experiment was initiated after human gastric adenocarcinoma cell lines SGC-7901 subcultured 24h. The effects of paclitaxel on SGC-7901 growth with different doses and time points was assessed by MTT assay, and so did the effects of P38 pathway inhibitor SB203580 with different doses, and the test doses were determined. RT-PCR method was used to detect COX-2 mRNA expression level of SGC-7901 cell li