小鼠最大灌胃量研究.docVIP

小鼠最大灌胃量研究 　　[摘要] 目的 研究小鼠最大灌胃量及其对小鼠心电图和心肌组织的影响。 方法 采用不同体重的小鼠灌胃后测定胃最大容量；以PEG6000制备不同黏度的溶液，以不同灌胃量灌胃后，记录小鼠心电图T波变化，制备小鼠心肌组织病理切片，光镜观察。 结果 在一定的体重范围内，小鼠体重与其灌胃量间存在一定的线性关系。与空白组比较，20 g/100 ml和40 g/100 ml两种黏度的PEG6000灌胃后小鼠心电图T波变化没有统计学意义（P0.05），小鼠心肌组织切片未见异常。 结论 可按线性关系推算小鼠的最大灌胃量；以最大灌胃量灌胃，在一定黏度范围内，小鼠的心电图及心肌组织未受影响。 　　[关键词] 小鼠；灌胃；最大耐受剂量 　　[中图分类号] R-33 [文献标识码] A [文章编号] 1674-4721（2015）01（b）-0004-03 　　Study on the maximum tolerant dosage of mice by gastric perfusion 　　YAN Hai-yan ZOU Chun-cai YANG Yang LI Ping DENG Xiao-yang 　　Pharmacy School of Wannan Medical College in Anhui Province，Wuhu 241002，China 　　[Abstract] Objective To study the maximum tolerant dosage of mice by gastric perfusion and examine the influence of the electrocardiogram and myocardial tissue. Methods The maximum capacity was determined by using different body weight of mice after gastric perfusion.PEG6000 solution with different viscosity was prepared，and the T wave changes in ECG were recorded after administrating various dosages by gastric perfusion.The pathological sections of myocardial tissue was prepared and observed by using light and electron microscopy. Results In a certain weight range，linear relationship exists between the weight of mice and the gastric volume.Compared with the blank group，T wave changes of ECG in mice after gastric perfusion with 20 g/ml and 40 g/ml PEG6000 solutions had no statistical difference（P0.05），and myocardial tissue sections in mice showed no abnormalities. Conclusion Maximum intragastric volume of mice can be calculated according to the linear relationship；It has no effect of ECG and myocardial tissue with the greatest amount of intragastic gavage in a certain solution viscosity range. 　　[Key words] Mice；Gastric perfusion；Maximum tolerant dosage 　　在口服药物的药理药效研究中常要求以最大给药体积给小鼠灌胃，并习惯性规定灌胃最大容量以20 g体重1 ml药量为限度[1-4]，有的以每鼠0.8 ml为最大限度[5-8]。这种习惯性用法经预试验确认，在多数情况下不会因灌胃量问题而导致小鼠死亡[9-12]，但所给药量是否是最大灌胃量值得商榷，灌胃量对小鼠生理指标（如心电图）及组织（如心肌组织）的影响也未见相关报道。 　　1 材料与方法 　　1.1 仪器 　　AUW-220D型电子天平（日本岛