小鼠胃和阑尾组织中hCD降解规律与死亡时间相关性研究.docVIP

小鼠胃和阑尾组织中hCD降解规律与死亡时间相关性研究 　　[摘要] 目的：观察小鼠死亡后不同时间胃和阑尾组织中h-CD含量的变化，探讨不同温度条件下h-CD的降解规律与死亡时间的关系及其组织差异性。方法：选取72只健康成年昆明小鼠，采用机械性窒息致死后，随机分为两组，分别置于4℃冰箱和25℃人工气候箱中，并于死后0、24、48、72、96、120 h提取胃及阑尾组织，采用免疫印记及计算机图像分析技术检测上述组织中h-CD的含量，应用SPSS 16.0软件对数据进行统计学分析。结果：①h-CD的含量随死亡时间的延长而降低，与死亡时间具有相关性；②不同温度条件下，胃和阑尾组织中h-CD的降解速度均存在明显差异（P＜0.05），h-CD在25℃组降解速度快于4℃组（P＜0.05）；③h-CD的降解速度在胃和阑尾组织中存在组织差异性，在阑尾组织中的降解速度较快（P＜0.05）。结论：h-CD的含量随死亡时间延长而降低，其降解速度受环境温度影响，温度升高，降解速度加快，且胃和阑尾组织中h-CD的降解速度具有组织差异性，其在阑尾组织中的降解速度快于胃组织中的降解速度。 　　[关键词] 法医病理学 重型钙调蛋白结合蛋白 死亡时间 免疫印记 　　[中图分类号] D919.4[文献标识码]A[文章编号]1673-7210（2011）02（c）-017-03 　　Associativity study between h-CD degradation regularity and the estimation of postmortem interval in stomach and appendix of mice 　　LIU Jia, LIANG Xinhua, JIAO Yan 　　(Faculty of Forensic Medicine of Shanxi Medical University, Taiyuan030001, China) 　　[Abstract] Objective: To observe the postmortem degradation of high molecular-weight caldesmon (h-CD) in stomach and appendix smooth muscle cells of mice, study the effects of postmortem intervals, and ambient temperature on the h-CD degradation of mice, and the variability of the h-CD degradation in stomach smooth muscle cells and appendix smooth muscle cells in different ambient temperature. Methods: 72 healthy adult Kunming mice were killed by asphyxia, and put them in 4℃ refrigerator and 25℃ artificial climate incubator respectively, then extractd the stomach and appendix tissues at 0, 24, 48, 72,96, 120 h after dead. The h-CD contents in the above tissues were quantitated by western-blot assay and collected by Image Pro Plus 5.0 image analysis system, then the datas were statistically analyzed with the SPSS 16.0 software. Results: ①h-CD content in stomach and appendix tissues decreased with the postmortem intervals, there was correlation between the postmortem degradation of h-CD and the postmortem intervals; ②The differences of the postmortem degradation of h-CD in different ambient temperature were significant (P＜0.05), the degradation