中药三七抗老年性痴呆活性部位的筛选及药理作用的分析-screening of anti - senile dementia active parts of panax notoginseng and analysis of its pharmacological effect.docxVIP

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中药三七抗老年性痴呆活性部位的筛选及药理作用的分析-screening of anti - senile dementia active parts of panax notoginseng and analysis of its pharmacological effect.docx

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中药三七抗老年性痴呆活性部位的筛选及药理作用的分析-screening of anti - senile dementia active parts of panax notoginseng and analysis of its pharmacological effect

Screening effective parts of Traditional Chinese Medicine Panax notoginseng and studying its pharmacological effects on Alzheimers disease ABSTRACT Objective: Screening effective parts of Panax notoginseng and studying its pharmacological effects on Alzheimers disease. Methods: (1)Screening effective parts of Panax notoginseng in vitro:MTT assay was adopted to study the proliferation of PC12 cell with treatments of diffrent parts of Panax notoginseng. (2)Anti-AD in vitro:MTT assay was used to detect the proliferation of PC12 cell induced by Aβ25-35 fragment treated with N-butanol extract of Panax notoginseng. (3)Acute toxicity experiment:mice was administrated intragastricly with maxium concentration and volume N-butanol extract of Panax notoginseng to determined the maximum tolerant dose. (4)Anti-AD effect in vivo:Ethologic examination was carried out using Morris water maze(MWM)to observe changes of the abilities of special learning and memeory in SAMP8.The content of SOD and GSH-PX was determined by Hydroxylamine method. The content of MDA was determined by TBA method.The contents of BACE-1,APP in brain tissue were assayed by reverse transcription polymerase chain reaction. Results: (1)MTT assay was used to filter out the N-butanol extract of Panax notoginseng can increase the PC12 cells. (2)Anti-AD in vitro:All of testing indexes in vitro experiment showed that the N-butanol extract of Panax notoginseng extract on AD cell model. (3)The tests of acute toxicity(MTD)showed that the maximal tolerance dose is 29.2g/kg which is 5840 times in adult daily dosage. (4)Anti-AD effect in vivo:All of the testing indexes in MWM experiment showed that the abilities of learning and memory in SAMP8 mice treated with the N-butanol extract of Panax notoginseng were remarkably improved as compared with model group and the group treated with medium dosage and high doseage N-butanol extract of Panax notoginseng was higher than the group with Huperzin A. (5)Spectrophotometry assay