成骨生长肽联合胰岛素样生长因子对成骨细胞增值影响.docVIP

成骨生长肽联合胰岛素样生长因子对成骨细胞增值影响 　　【中图分类号】R580 【文献标识码】A 【文章编号】1005-2720(2009)01 - 016 - 03 　　【摘要】目的 探讨成骨生长肽(OGP)联合胰岛素样生长因子(IGF)对体外培养成骨细胞增值分化的影响。方法:将鼠成骨细胞分别与OGP(10-10mmol/L)、IGF-1(100ng/ml)、OGP(10-10mmol/L )+ IGF-1(100ng/ml)共同培养,于1、3、5d分别进行3H-TdR、3H-Tproline的掺入量与单独使用因子组、对照组相比,在各个时段都存在显著性差异(P<0.01);OGP联合IGF组对碱性磷酸酶合成量与对照组、OGP 、IGF组相比有显著性差异(P<0.01)。结论:OGP联合IGF可显著促进成骨细胞DNA、胶原蛋白、碱性磷酸酶的合成,对促进成骨细胞增值分化有协同作用。 　　【关键词】成骨细胞;体外培养;成骨生长肽;胰岛素样生长因子;细胞增值与分化 　　Effect of OGP combination with IGF on proliferation and differentiation of Cultured rat osteoblast 　　Cui kai Ma xun 　　(Orthopedics department,the second hospital of ShanXi Medical University . TaiYuan,030001) 　　【Abstract】Objective To discuss effect of OGP combination with IGF-1 on proliferattion of cultured rat osteoblast.Methods The second subcultures were exposed to OGP(10-10mmol/L)、IGF-1(100ng/ml)and IGF-1(100ng/ml) combination withIGF(100ng/ml).The DNA and collagen protein synthesis were determined by theIncorporation of 3H-TdR, 3H-Tproline of osteoblast respectively at 1st、3rdand5th days。Alkaline phosphatase activity in the media collected at lst、3rdand5th days of culture was determined with king-armstrong technique 。Results The incorporation of 3H-TdR and 3H-Tproline of osteoblast in OGP combination with IGF-1 group was statistically different at the same period(P<0.01)from that in the control group、the OGP group and the IGF-1group.Conclution The combination OGP with IGF-1 can significantly stimulate the synthesis of DNA,collagen protein and alkaline phosphatase ofOsteoblasts,and has a promoting effect on proliferation and differentiation of osteoblasts. 　　【Key words】Osteoblast;in vitro culture;Osteogenic Growth Peptide;Insulin-like growth factor-1;Cell proliferattion and differentiation 　　 　　有学者研究发现骨髓损伤后早期修复过程中成骨活动增强,并在血清中检测到一种新的促进有丝分裂的生长因子-成骨生长肽(Osteogenic Growth Peptide)[1]。胰岛素样生长因子是一类具有胰岛素样生物活性的多肽,既具有胰岛素样作用,又可促进细胞增值和分化[2]。在骨折愈合过程中,成骨细胞起了至关重要的作用,而成骨细胞的增值分化又是在多种细胞因子的影响下完成,但就其具体机制及细胞因子间相互作用关系尚不完全清楚,因此,本实验在鼠成骨细胞分离培养的基础上,