转hgm-csf基因hepg2细胞疫苗侵袭性和生长活性初步检测-preliminary detection of invasiveness and growth activity of hgm - csf transgenic hepg 2 cell vaccine.docxVIP

PAGE PAGE 10 The Initial Detection of HGM-CSF Gene Transfected HepG2 Cell Vaccine’s Invasion and Growth Activity ABSTRACT Objective: In this study through researching the invasive gene expression changes、the number of cancer cell which were throughed the basement membrane and growth activity in vitro of hepG2 liver cancer vaccines which were treatmened by 60Co,we want to verify initially the invasion and growth activity changes of liver cancer vaccine. Methods: Culturing three hepatoma cells in vitro (A.wild-type HepG2 hepatoma cells ;B.transfected with hGM-CSF gene in HepG2 hepat