放线菌P32抗菌活性筛选及发酵液稳定性研究.docVIP

放线菌P32抗菌活性筛选及发酵液稳定性研究 　　摘要对贵州高坡地区不同类型的土壤中分离出的256株放线菌进行发酵液拮抗性筛选,得到1株放线菌菌株P3-2。采用菌丝生长速率法,对其抗菌活性进行研究,并在不同条件下测定发酵液的稳定性,结果表明,放线菌P3-2菌株发酵液对14种供试病原菌均具有不同程度的抑制作用,其10倍稀释液对其中9种病原真菌菌丝生长的抑制率达到85%以上,尤其对油菜菌核病菌和黄瓜灰霉病菌的抑菌效果最好,抑制率达到100%。发酵液的稳定性测定结果表明,发酵液对热、酸和紫外线均较稳定,对碱稳定性较差。该菌株的代谢产物具有广谱抑菌活性和较强的稳定性,具有一定的研究开发价值。 　　关键词放线菌;抗菌活性;发酵液;筛选;稳定性 　　中图分类号Q939.13文献标识码A文章编号 1007-5739(2010)06-0016-02 　　 　　StudyonSelectionofAntibioticActivitiesandStabilityofFermentationBrothofActinomyceteP3-2 　　WANG Jang-fengZHANG ling-lingHUANG Jian* 　　(Center for Research and Development of Fine Chemicals,Key Laboratory of Green Pesticide and Bioengineering,Ministry of Education,Guizhou University,Guiyang Guizhou 550025) 　　Abstract256 actinomycetes were isolated from different soil collected from Gaopo area in Guizhou province and one strain P3-2 was got.By means of mycelium growth rate,the antibiotic activities and stability of fermentation broth of strain P3-2 were studied.The antimicrobial test showed that:the inhibition rate of all the 14 strains were different and 9 of them was more than 85% when fermentation broths was diluted by 10 times.Organize method tests showed that inhibition rate of fermentation broths of the strain P3-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100%.Through the research of stability of fermentation broths of actinomycete strain P3-2 to heat,acid,alkali and light,it had a stable activity to heat,acid and UV,but unstable to alkali.Antimicrobial spectrum of actinomycete strain P3-2 was widely and antimicrobial activity of fermentation broths was stable.The actinomycete strain P3-2 had some research value and was worth developing. 　　Key wordsactinomycete;antibiotic ctivity;fermentation;selection;stability 　　 　　近年来,微生物源农药得到越来越多的关注,研制能防治各种农作物病虫害及作为除草剂的无公害农用抗生素,研制能防治家畜、家禽等疾病和促进其生长的畜用抗生素,是目前抗生素的一个重要的发展方向。抗生素的重要来源是微生物,而目前从微生物中发现的大约8 000种活性物质中又有近80%是由放线菌所产生。因此从放线菌代谢产物中寻找新的农用抗生素成为当前国内外的一个研究热点。该研究是从贵州各地土壤中分离放线菌,从中筛选出对多种农作物病原菌有显著作用的拮抗菌。笔者筛得编号为P3-2的菌株,其对14种病原菌都有显著的拮抗作用,并对发酵