文冠果种子发育过程中神经酸合成积累相关基因表达分析.docVIP

文冠果种子发育过程中神经酸合成积累相关基因表达分析 　　摘要：利用半定量RT-PCR技?g分析比较文冠果（Xanthoceras sorbifolium Bunge）种子发育过程中神经酸合成相关基因（KCS、KCR、HCD和ECR基因）在文冠果高神经酸（HNA）、低神经酸（LNA）品系的表达模式，旨在为解析文冠果神经酸合成与积累的分子机制提供重要参考。结果表明，4个基因在两个品系花后40 d的种胚中表达量最高，在随后的种胚发育过程中表达量呈下降趋势或表达量极低。在花后40 d时KCS基因在HNA品系中的表达量高于LNA，但花后54、68和81 d时在HNA品系中的表达量低于LNA。与KCS基因不同的是，HCD基因在HNA品系花后40 d种胚中的表达量低于LNA，而在花后54、68和81 d时HNA品系中的表达量高于LNA。KCR基因在HNA品系种胚中的表达量均低于LNA。ECR基因在HNA品系花后40和82 d种胚中的表达量高于LNA，表明花后40 d是种胚发育过程中神经酸积累的关键时期。KCS和ECR基因主要在种胚发育的早期（花后40 d）调控神经酸的合成，HCD基因在种胚发育的中期和后期调节神经酸的合成过程。 　　关键词：文冠果（Xanthoceras sorbifolium Bunge）；种胚；神经酸；KCS基因；半定量RT-PCR；表达分析 　　中图分类号：S794.9；Q786 文献标识码：A 文章编号：0439-8114（2018）04-0117-04 　　DOI：10.14088/j.cnki.issn0439-8114.2018.04.030 　　Expression of Genes Involved in Biosynthesis and Accumulation of Nervonic Acid during Seed Development in Xanthoceras Sorbifolium Bunge 　　WANG Li，LIU Xiao-hui，MA Jing-yi，LUO Yuan，LI Zhi-min 　　（College of Environment and Resources，Dalian Minzu University，Dalian 116600，Liaoning，China） 　　Abstract： Expression patterns of the genes（KCS，KCR，HCD and ECR gene） involved in biosynthesis and accumulation of nervonic acid were studied and compared in high and low nervonic acid-yielding Xanthoceras sorbifolium Bunge during embryonic development using semi-quantitative RT-PCR technology. This will play an important role for understanding molecular mechaisms of biosynthesis and accumulation of nervonic acid in X. sorbifolium. The results showed that the expression levels of four genes were highest in embryos of two lines at the 40 days after anthesis compared to that of other developmental stages，and then the expression levels decreased or was very low during the subsequent embryo development. The expression level of KCS gene was higher in HNA line than LNA line at 40 d，but its expression level was lower in HNA line compared to LNA line at 54，68 and 81 d. In contrast to the KCS gene，the expression level of HCD gene was lower in HNA line than LNA line at 40 daa，whereas its expression level was