木糖醇对黏性放线菌生长及产酸影响体外研究.docVIP

木糖醇对黏性放线菌生长及产酸影响体外研究 [文章编号] 1000-1182(2014)03-0217-04 　　[摘要] 目的 对比不同质量浓度下木糖醇对黏性放线菌生长及产酸的影响。方法 分别用含不同质量浓度（128、64、32、16、8、4 g?L-1）木糖醇的脑心浸液（BHI）液体培养基在厌氧条件下培养黏性放线菌，测定其最小抑菌质量浓度（MIC）；然后测量对照组以及1/2、1/4、1/8 MIC和MIC质量浓度时培养1.5、3、6、12、24、48 h液体培养基的pH值，计算ΔpH值，同时测量2、4、6、8、10、12 h液体培养基的光密度（OD550）值；最后测定抑制50%及 90%黏性放线菌生物膜形成的最低木糖醇质量浓度（即MBIC50和MBIC90）。运用SPSS 19.0进行数据分析。结果 木糖醇能抑制黏性放线菌的生长，MIC为64 g?L-1。培养12 h后，各组之间的ΔpH值差异均有统计学意义（P0.05），OD550值随MIC质量浓度的增加而减小。提示黏性放线菌在含1/2 MIC及MIC的木糖醇培养基里生长及产酸能力随木糖醇质量浓度的升高而下降。木糖醇的MIBC50为64 g?L-1，MIBC90为128 g?L-1，说明木糖醇培养基能抑制黏性放线菌生物膜的形成。结论 木糖醇能有效地抑制黏性放线菌的生长、黏附以及产酸，有一定的抑制致龋菌和防治龋病的效果。 　　[关键词] 木糖醇；黏性放线菌；生长；产酸 　　[中图分类号]R 780.2 [文献标志码] A [doi] 10.7518/hxkq.2014.03.002 　　 　　In vitro study of xylitol on the growth and acid production of Actinomyces viscosus Guo Houzuo, Xiao Yao, Lian Xiao-tian, Zou Ling. (State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China) 　　[Abstract] Objective This research aimed to study the inhibitory effect of xylitol on the growth and acid production of Actinomyces viscosus (A.#8198;viscosus). Methods We cultivated A.#8198;viscosus in anaerobic conditions with different concentra-tions (128, 64, 32, 16, 8, and 4 g?L#8722;1) of xylitol brain heart infusion liquid medium and determined the minimum inhibitory concentration (MIC). Subsequently, we measured the pH value of the control group, as well as those of 1/2, 1/4, 1/8 MIC, and MIC concentration groups at 1.5, 3, 6, 12, 24, and 48 h. The ΔpH and OD550 at 2, 4, 6, 8, 10, and 12 h were calculated. We discovered that the minimum xylitol concentrations suppressed 50% and 90% A.#8198;viscosus biofilm formation (i.e., MBICshy;50 and MBIC90). SPSS 19.0 was used to analyze the collected data, and conclusions were drawn afterward. Results Xylitol inhibited the growth of A.#8198;viscosus at MIC of 64 g?L-1. After 12 h, the differences of pH value among groups were all statisti-cally significant (P0.05), and OD550 also increased wh