核酸血筛标本冷链运输保存方法和效果分析.docVIP

核酸血筛标本冷链运输保存方法和效果分析 　　[摘要] 目的 探讨血站的核酸标本冷链运输保存的方法和效果。方法 按照《血站技术操作规程（2015版）中标本运输的要求，模拟在车辆运输的条件下，使用不同的保存方法，测试运输箱内标本的保温时间（即标本运输温度保持在2～10℃的时间）。研究共分3组，第1组：标本运输箱底部放置一块4℃冷源板；第2组：标本运输箱底部放置一块冷源板，四周随机选取两面各放一块冷源板，共三块冷源板；第3组：标本运输箱底部放一块冷源板，四周各放一块冷源板，共五块冷源板。结果 模拟车内开空调环境，调节实验室内温度为23～27℃，湿度为30%～70%，第1、2、3组运输箱保温时间分别为：（0.16±0.02）h、（6.16±0.37）h和（13.78±0.71）h，两两比较差异均有统计学意义（F=2197.474，P0.05）。结论 一块冷源不能满足核酸标本运输的要求，摆放3块冷源和5块冷源，可以确保核酸标本运输保存的温度在适宜范围内，为输血安全提供保障。 　　[关键词] 血液中心；核酸标本；运输与保存；冰源 　　[中图分类号] R446.11 [文献标识码] A [文章编号] 2095-0616（2017）07-128-03 　　The method and effect analysis of cold chain transportation of 　　nucleic acid sieve 　　YAO Junqing YE Huifen 　　Dongguan Blood Center， Dongguan 523000， China 　　[Abstract] Objective To investigate the ways and effects of blood sample cold chain transportation preservation in the blood center. Methods According to the requirements of Blood Stations Technology Operation Procedures （2015 edition）， the holding time of the samples in the transport cases （the time of samples transport temperature holding between 2 to 10℃） were tested by different preservation methods， simulated the condition of transportation by vehicles. The samples were divided into three groups. The first group were used one piece of cold source plate at the bottom of the transport cases .The second groups were used three pieces of cold source plate， one piece were placed at the bottom， and the others were placed at two sides selected randomly. The third groups were used five pieces， one placed at the bottom， and the others were placed at each side. Results To simulate the condition in vehicles with air condition to regulate the temperature of a laboratory to 23-27℃， humidity to 30%-70%，the holding time in the first， the second and the third groups were （0.16±0.02）h、（6.16±0.37）h and （13.78±0.71）h， respectively. There was significant difference between the two groups （F=2197.474， P0.05）. Conclusion One piece of cold source cannot reach the requirement of nucleic acid samples tran