栽培环境对铁皮石斛生长与代谢成分影响お.docVIP

栽培环境对铁皮石斛生长与代谢成分影响お 　　[摘要]?槊魅吩耘嗷肪扯蕴?皮石斛的生长及代谢成分的影响，对铁皮石斛C13品系在岩壁附生、梨树附生和设施盆栽3种栽培环境下进行形态特征、显微结构、多糖和浸出物含量的观察与分析。结果表明，生长环境显著地影响铁皮石斛的生长，在农艺性状上，岩壁附生和梨树附生的铁皮石斛叶片稀少、颜色由绿变紫红、伸展角度减小，茎秆多呈紫色、较粗壮，茎长分别为34，40 cm，普遍比设施盆栽（75 cm）短，且根系特别发达；在显微结构上，野外生长的铁皮石斛与设施栽培比均表现出叶片增厚、上表皮厚度增加、下表皮表皮毛丛生、气孔小而密，根被细胞、外皮层、内皮层的细胞壁均偏厚，且根被、外皮层、内皮层、髓部的细胞小且排列紧密，但栽培环境没有产生特异性的组织结构，主要是结构参数大小和数量的变化，说明铁皮石斛具有很好的环境适应能力；此外，生长环境显著地影响铁皮石斛多糖和醇溶性浸出物等代谢成分的积累，岩壁附生铁皮石斛多糖和醇溶性浸出物含量最高，质量分数分别达3734%，1166%，梨树附生次之，质量分数分别为3390%，962%，均高于设施盆栽；通过HPLC分析，岩壁附生铁皮石斛中醇溶性浸出物成分比另外2种栽培模式更加复杂，说岩壁附生栽培环境更有利于铁皮石斛次生代谢产物的合成和积累。 　　[关键词]铁皮石斛； 栽培环境； 形态特征； 显微结构； 代谢成分 　　Effects of cultivation environments on Dendrobium catenatum 　　LIN Yikai1， ZHU Yuqiu2， SI Jinping1*， QIN Lang2， ZHU Yan3，WU Lingshang1， LIU Jingjing1 　　（1State Key Laboratory of Subtropical Silviculture， Zhejiang A F University， Lin′an 311300， China； 　　2 Anhui Mulongshan Ecotourism Development Co， Ltd， Quanjiao 239500， China； 　　3Hangzhou Zhenheng Biotechnology Co， Ltd， Lin′an 311300， China） 　　[Abstract]The study was aimed to clarify the effect of three cultivation environments on the growth and metabolism of Dendrobium catenatum C13 group There were three different cultivation conditions including rock epiphytic cultivation， pear epiphytic cultivation and pot cultivation Morphological characteristics and agronomic characters of D catenatum were observed and measured Microstructure， contents of polysaccharide and alcoholsoluble extracts were measured by paraffin section method， phenolsulfuric acid method and hotdip method， respectively The result showed that the cultivation environment significantly affected the growth of D catenatum， the leaves of D catenatum that cultivated on the rock and pear were sparse and small， the stems were short and purple and the root system was developed Compare with potted cultivation， D catenatum from rock epiphytic cultivation and pear epiphytic cultivation showed the following characteristics in the microstructure： the upper epidermis became thick