液基细胞学HPV―DNA及hTERC基因联合检测在宫颈癌筛查中应用价值.docVIP

液基细胞学HPV―DNA及hTERC基因联合检测在宫颈癌筛查中应用价值 　　[摘要] 目的 探讨TCT、HPV-DNA及hTERC基因联合检测在宫颈癌筛查中的应用价值。 方法 对480例筛查者分别进行TCT、HPV-DNA和hTERC基因检测，并以病理学检测结果为参照标准，对三种检查方法的敏感度、特异度及符合率进行分析。 结果 480例受检者中，TCT检测异常49例，HPV阳性54例，hTERC基因扩增阳性29例。单一筛查时，TCT灵敏度最高，hTERC特异度和符合率最高；联合筛查时，TCT+HPV（+）灵敏度最高，HPV+hTERC（+）特异度和符合率最高，单项筛查和联合筛查的检出率均与病理级别有关（P0.05）。 结论 联合检查应用于宫颈癌筛查中，优势较为明显，其中以HPV-DNA联合hTERC基因检测效果最为理想，检查结果均与宫颈高级别病变存在密切的关系。 　　[关键词] 宫颈癌；HPV-DNA；hTERC基因；联合检测；早期诊断 　　[中图分类号] R737.3 [文献标识码] A [文章编号] 1673-9701（2016）17-0009-04 　　The application value of combined detection of TCT， HPV-DNA and hTERC genes in screening of cervical cancer 　　ZHAO Runzhen 　　Department of Pathology， Fenyang Hospital of Shanxi Province， Fenyang 032200， China 　　[Abstract] Objective To study the application value of combined detection of TCT， HPV-DNA and hTERC genes in screening of cervical cancer. Methods 480 subjects were examined by TCT testing， HPV-DNA and hTERC genes detection. The sensitivity， specificity and coincidence rate of the three methods were studied with the results of pathological examination as the reference standard. Results In 480 subjects， abnormalities were found in 49 cases by using TCT testing， further， 54 cases were HPV-positive， and amplification positive PCR product of hTERC gene were detected in 29 cases. Single-arm screening results indicated that TCT had the highest sensitivity， and hTERC had the highest specificity and coincidence rate； by combined screening， the sensitivity of TCT+HPV（+） was the highest， while the HPV+hTERC（+） had the highest specificity and coincidence rate. Both single-arm screening and combined screening were correlated with histological grades， with statistical significance（P0.05）. Conclusion Combined examination has a remarkable advantage for the screening of cervical cancer over the single-arm screening， and the detection effect of HPV-DNA combined with hTERC gene is most evident. Besides， the inspection results are closely correlated the high levels of cervical lesions. 　　[Ke