甜樱桃MYB转录因子基因克隆与表达分析.docVIP

甜樱桃MYB转录因子基因克隆与表达分析 　　摘要：本研究利用转录组测序结果，通过RT-PCR从甜樱桃品种‘美早’果实中克隆出1个MYB转录因子基因，将其命名为PaMYB10，GenBank登录号为ALH21137.1。该基因编码的氨基酸序列具有R2和R3保守结构域，属于R2R3-MYB家族基因，与蔷薇科的桃、欧李、梅等作物亲缘关系较近。qRT-PCR结果表明，PaMYB10在红色品种‘美早’的茎、叶、花和果实中均有表达，但表达量存在差异，在成熟果实中表达量最高。‘美早’中花青苷含量随着果实的发育逐渐升高，PaMYB10的表达显著上调；‘13-33’中花青苷含量随着果实的发育变化不明显，基因表达量变化也不明显。在果实发育后期，‘美早’果实中花青苷含量和PaMYB10的表达量均显著高于‘13-33’。推?yPaMYB10的高水平表达可能与红色甜樱桃果实花青苷积累有关。 　　关键词：甜樱桃；花青苷；转录因子；MYB；基因表达 　　中图分类号：S662.5：Q781文献标识号：A文章编号：1001-4942（2018）03-0006-05 　　Abstract Based on our previous transcriptome data of sweet cherry fruit， the MYB transcription factor gene was cloned using RT-PCR method from sweet cherry cultivar ‘Tieton’， and named PaMYB10. The sequence was submitted to GenBank with the accession number of ALH21137.1. Sequence analysis revealed that the deduced amino acid of PaMYB10 contained R2 and R3 conserved domain and it was a typical R2R3-MYB family gene. The encoded protein PaMYB10 had the closest genetic relationship with Prunus persica， Prunus domestica and Prunus mume. The result of qRT-PCR showed that PaMYB10 was expressed in stem， young leaf， flower and fruit tissues in red cultivar ‘Tieton’， but the expression quantity was different. The expression quantity of PaMYB10 was the highest in the mature fruit of ‘Tieton’. The content of anthocyanin in ‘Tieton’ increased gradually and the expression level of PaMYB10 showed significantly up-regulated along with the fruit development. However， the content of anthocyanin and expression level of PaMYB10 in the yellow cultivar ‘13-33’ were almost unchanged and remained at a very low level during fruit ripening. In the later stage of fruit development， the content of anthocyanin and expression level of PaMYB10 in the red cultivar ‘Tieton’ were significantly higher than those of the yellow cultivar ‘13-33’. It is speculated that the high level expression of PaMYB10 may associated with the anthocyanin accumulation in red sweet cherry fruit. 　　Keywords Sweet cherry； Anthocyanin； Transcription