生髓育麟汤对实验性少弱精子大鼠附睾精子质量影响.docVIP

生髓育麟汤对实验性少弱精子大鼠附睾精子质量影响 　　[摘要] 目的 观察生髓育麟汤对少、弱精子大鼠附睾精子质量的影响。 方法 60只大鼠按照常规饲养方法饲养，1周后随机抽出10只大鼠编入正常对照组，另外50只予雷公藤多苷片20 mg/（kg?d）灌胃26 d。造模完毕后随机抽取10只编入造模组处死，将剩下的40只大鼠随机分为4组：生髓育麟汤高剂量组[20 mL/（kg?d）]、低剂量组[10 mL/（kg?d）]、五子衍宗丸组以及不给予任何药物，只给予正常食物和水的观察对照组，每组各10只。实验结束后获取大鼠附睾精子对各组大鼠的精子质量进行分析。 结果 生髓育麟汤能明显增加精子密度、前向运动精子比例、精子活动率，并且随着浓度的增加，效果更显著，此外，生髓育麟汤还能改善精子运动特征参数；生髓育麟汤低剂量组、生髓育麟汤高剂量组与观察对照组比较，差异均有高度统计学意义（P 0.01）。 结论 生髓育麟汤能改善少、弱精子大鼠附睾中的精子质量。 　　[关键词] 生髓育麟汤；少、弱精子症；大鼠；动物实验 　　[中图分类号] R271.14 [文献标识码] A [文章编号] 1673-7210（2016）04（b）-0022-05 　　[Abstract] Objective To study the effect of Shengsui Yulin Decoction on the sperm quality in rats with oligospermatism. Methods Sixty rats were fed by normol way for a week， then fifty rats oligospermatism models been made by GTW. Ten rats were killed as models after molding， the other 40 rats were equally divided into four groups： the high-dose Shengsui Yulin Decoction group [20 mL/（kg?d）]， the low-dose Shengsui Yulin Decoction group [10 mL/（kg?d）]， WuZiYanZong Tablets group and the observation control group only feed by water and food. The sperm from the rats epididymis was extracted and sperm quality of each group was detected. Results Sperm density， proportion of progressive sperm， spermatozoal motivity were increased by Shengsui Yulin Decoction， and the therapeutic effect was increased with the increasing of the concentration of Shengsui Yulin Decoction， and the sperm motion parameters were improved by Shengsui Yulin Decoction； compared with observation control group， those indexes of the high-dose Shengsui Yulin Decoction group and the low-dose Shengsui Yulin Decoction group had statistically significant difference （P 0.01）. Conclusion Shengsui Yulin Decoction can improve the sperm quality of the rat testis with oligospermatism. 　　[Key words] Shengsui Yulin Decoction； Oligospermatism； Rat； Zoopery 　　不育症是男性常见疾病，也是影响夫妻感情、家庭稳定的重要原因[1]。据世界卫生组织（WHO）调查表明：男性精子质量从20世纪中期开始呈下降趋势，目前已成为第三大疾病，仅次于心脑血管病和肿瘤[2]。早在1998年就有学者统计发现全球共有8000万对夫妇患不育症，并以每年200万对的速度递增，其