瓜蒌薤白半夏汤对ApoE小鼠血脂代谢氧化应激和主动脉Lox―1表达影响.docVIP

瓜蒌薤白半夏汤对ApoE小鼠血脂代谢氧化应激和主动脉Lox―1表达影响 　　[摘要] 观察瓜蒌薤白半夏汤（GXBD）在调节血脂代谢、抗氧化、干预ox-LDL/Lox-1通路的作用，探讨其抗动脉粥样硬化（AS）的作用机制。以高脂饲料饲喂雄性Apo-E-/-小鼠42只，建立AS模型。AS模型小鼠随机分为模型组、辛伐他汀组、GXBD高、低剂量组，以C57BL/6J雄性小鼠作为正常对照组；每组10只，连续灌胃给药8周。末次给药后24 h，检测血清TC，TG，LDL-C，HDL-C及SOD，MDA，GSH-px，ox-LDL水平；HE染色?^察主动脉组织结构变化，Western blot和PCR分别检测主动脉Lox-1蛋白表达和mRNA水平。结果显示模型组小鼠血脂指标含量及血清MDA，ox-LDL水平显著高于正常对照组，SOD，GSH-px显著低于正常对照组，主动脉Lox-1蛋白表达水平及其mRNA水平显著高于正常对照组（P0.05），模型组主动脉出现明显粥样硬化斑块；GXBD高、低剂量组和辛伐他汀组小鼠血脂指标含量及MDA，ox-LDL水平显著低于模型组，SOD，GSH-px显著高于模型组，主动脉Lox-1蛋白表达水平及其mRNA水平显著低于模型组（P0.05），主动脉组织粥样硬化病变明显减轻。说明调节血脂代谢，抗氧化、抑制Lox-1的表达、干预ox-LDL/Lox-1通路，可能是其抗AS的机制之一。 　　[关键词] 瓜蒌薤白半夏汤； 动脉粥样硬化； 抗氧化； 血脂； ox-LDL/Lox-1通路 　　[Abstract] To observe the functions of Gualou Xiebai Banxia decoction（GXBD） on regulating lipid metabolism， anti-oxidation， and interposing ox-LDL/Lox-1 pathway， and to explore its anti-atherosclerosis （AS） mechanisms. AS models were established by using 42 Apo-E-/- male mice with high fat diet. AS model mice were randomly divided into the model group， simvastatin group， and GXBD high and low dose groups. C57BL/6J male mice were used as the normal control group， n=10 and the treatment lasted for 8 weeks. The levels of TC， TG， LDL-C， HDL-C， SOD， MDA， GSH-px， and ox-LDL in blood serum were tested 24 h after the last administration. The changes of aortic tissues structure were observed by HE staining； the expression levels of Lox-1 protein and the expression levels of mRNA were detected by Western blot and PCR respectively.Results showed that the blood lipid levels and MDA， ox-LDL levels in blood serum of model group were significantly higher than those in the normal control group， but SOD， GSH-px levels were significantly lower than those in the normal control group， and the Lox-1 protein and mRNA expression levels were also significantly higher than those in the control group（P 　　[Key words] Gualou Xiebai Banxia decoction； atherosclerosis； antioxidant； blood lipid； ox-LDL/Lox-1 pathway 　　动脉粥样硬化（atherosclerosis，AS）是冠