生长抑制因子1在肝细胞肝癌中表达及临床意义.docVIP

生长抑制因子1在肝细胞肝癌中表达及临床意义 　　[摘要] 目的 探?生长抑制因子ING1（inhibitor of growth1）在肝细胞肝癌（human hepatocellular carcinoma，HCC）中的表达情况及临床意义。 方法 采用定量RT-PCR方法，检测86例HCC组织及相应癌旁组织中ING1 mRNA的表达量，分别计算肿瘤组织和癌旁组织的△CT值，来计算△CT癌旁/△CT肿瘤的比值，以2为阈值，分成高表达组和低表达组，分析两组患者的临床资料及生存时间。 结果 ING1 mRNA在HCC组织中的表达水平明显低于癌旁肝组织（P0.05）。高表达组和低表达组患者的中位生存时间分别为34.5个月和19.5个月，高表达组明显长于低表达组（P0.05）。 结论 ING1作为抑癌基因可能参与了HCC的发生发展过程，可作为判断HCC预后的重要分子标志。 　　[关键词] 抑癌基因；肝细胞肝癌；生长抑制因子1；实时荧光定量PCR 　　[中图分类号] R735.7 [文献标识码] A [文章编号] 1673-9701（2016）35-0001-04 　　Expression of inhibitor of growth 1 in human hepatocellular carcinoma and its clinical significance 　　ZHANG Lei1 CHEN Yunzhi2 CHEN Yongping1 　　1.Department of Infectious Diseases， the First Hospital Affiliated to Wenzhou Medical University， Wenzhou 325000，China；2.Department of Hepatobiliary Surgery， the First Hospital Affiliated to Wenzhou Medical University， Wenzhou 325000， China 　　[Abstract] Objective To discuss the expression and clinical significance of inhibitor of growth 1 （ING1） gene in human hepatocellular carcinoma（HCC）. Methods There were 86 pairs of samples comprised of HCCs and their paracancerous tissues. The mRNA levels of ING1 in these samples were detected by quantitative RT-PCR. The △CT values and the ratio of △CT values within each pair were calculated respectively. The different pairs were grouped based on 2 as the cut-off value of the ratio. Correlations between clinicopathologic variables， over all survival and ING1 mRNA expression were analyzed. Results ING1 mRNA was significantly lower in HCC than that in the paired paracancerous tissues（P0.05）. Survival time of high expression group was significantly longer than that of low expression group（median survival， 34.5 months vs 19.5 months，P 　　[Key words] Tumor suppressor gene； Human hepatocellular carcinoma； Inhibitor of growth 1； Quantitative RT-PCR 　　近年来发现生长抑制因子（inhibitor of growth，ING）家族能诱导细胞生长周期停滞、促进细胞衰老和凋亡、调控DNA复制及损伤应答、抑制肿瘤的发生与发展[1]。已有研究证实，此类基因在肿瘤组织中的表达大多呈下调状态[2]。生长抑制基因1（inhibitor of growth 1 gene，ING1）作为I