糖尿病视网膜病变患者氧化应激状态及炎症因子临床研究.docVIP

糖尿病视网膜病变患者氧化应激状态及炎症因子临床研究 　　[摘要] 目的 通过测定糖尿病视网膜病变（DR）患者血清晚期蛋白氧化产物（AOPP）、血管内皮细胞生长因子（VEGF）及色素上皮衍生因子（PEDF）的水平，探讨其在DR中的可能作用。 方法 选择2型糖尿病（T2DM）患者90例，分为T2DM无DR（NDR）组30例，背景期DR（BDR）组30例，增殖期DR（PDR）组30例。同时选择健康对照组30例。用Wikto-sarsat介绍的方法改进后测定各组血清AOPP水平，酶联免疫吸附（ELISA）法测定血清VEGF及PEDF水平。 结果 随着DR的进展，各组血清中AOPP水平逐渐升高，差异均有统计学意义（P0.05）。与正常对照组比较，2型糖尿病各组VEGF水平均显著升高（P0.05）；BDR组及PDR组与NDR组比较，VEGF水平均明显高于NDR组（P0.05）；但PDR组VEGF含量较BDR组有所下降（P0.05）。各组血清中PEDF水平逐渐下降，差异均有统计学意义（P0.05）。Pearson相关分析表明血清VEGF与PEDF水平呈负相关（r=-0.734，P0.05）；而血清AOPP与VEGF水平呈正相关（r=0.816，P0.05），与PEDF水平呈负相关（r=-0.905，P0.05）。 结论 糖尿病患者血清AOPP和VEGF水平的升高，PEDF水平的下降可能促进了DR的发生发展。 　　[关键词] 糖尿病视网膜病变；晚期蛋白氧化产物；血管内皮细胞生长因子；色素上皮衍生因子 　　[中图分类号] R587.2 [文献标识码] B [文章编号] 1673-9701（2015）35-0029-03 　　[Abstract] Objective By measuring the level of serum advanced oxidation protein products （AOPP）， vascular endothelial growth factor （VEGF） and pigment epithelium-derived factor （PEDF） in patients with diabetic retinopathy（DR）， and to explore its possible role in the pathogenesis of DR. Methods 90 type 2 diabetes mellitus （T2DM） patients were divided into three groups： 30 patients with non-DR （NDR）， 30 patients with back ground-DR （BDR）， 30 patients with proliferative-DR （PDR）. At the same time， 30 healthy controls were selected. The concentration of serum AOPP was measured by modified Wikto-Sarsat， while VEGF and PEDF by ELISA assay. Results The serum level of AOPP increased gradually along with the development of DR， and the difference was statistically significant （P0.05）. Serum levels of VEGF in T2DM groups were significantly increased compared with the control group （P0.05）， and VEGF in BDR and PDR groups were significantly higher than that in NDR group （P0.05）， but the serum level of VEGF in PDR group was significantly lower than that in BDR group （P0.05）. The serum level of PEDF in each group was gradually decreased， and the difference was statistically significant（P0.05）. Pearson correlation analysis showed that the serum level of VEGF was negatively co