纳洛酮对油酸型ARDS大鼠血清中心房钠尿肽B型脑钠肽及肺组织Na―K―ATP酶含量影响研究.docVIP

纳洛酮对油酸型ARDS大鼠血清中心房钠尿肽B型脑钠肽及肺组织Na―K―ATP酶含量影响研究 　　[摘要] 目的 研究纳洛酮对油酸型急性呼吸窘迫综合征（ARDS）大鼠的血清中心房钠尿肽、B型脑钠肽及肺组织Na+-K+-ATP酶含量的影响。 方法 2014年1月～2015年1月将30只健康SD大鼠随机分为正常组、ARDS模型组、纳洛酮治疗组，由股静脉缓慢注入油酸0.1 mL/kg建立ARDS模型，测定血清中心房钠尿肽（ANP）、B型脑钠肽（BNP）、肺组织Na+-K+-ATP酶（NKA）含量，计算左肺湿/干重比值，制备光镜标本和电镜标本观察肺组织。 结果 ARDS模型组与正常组比较，左肺湿/干重比值、血清ANP、BNP含量显著升高，肺组织NKA含量显著下降（P0.01），肺组织出现明显病理损害，与ARDS模型组比较纳洛酮治疗组的变化较轻（P0.01），肺组织变化较轻。 结论 纳洛酮可抑ANP、BNP升高，提高肺组织Na+-K+-ATP酶活性，纳洛酮对实验性大鼠油酸型急性呼吸窘迫综合征有一定的治疗作用。 　　[关键词] 纳洛酮；急性呼吸窘迫综合征；油酸；心房钠尿肽；B型脑钠肽 　　[中图分类号] R563.8 [文献标识码] A [文章编号] 1673-9701（2016）15-0023-03 　　[Abstract] Objective To study the influence of naloxone on serum atrial natriuretic peptide（ANP） and B-type natriuretic peptide（BNP）， and Na+-K+-ATP enzyme（NKA） content in lung tissues in oleic acid induced acute respiratory distress syndrome （ARDS） rats. Methods From January 2014 to January 2015， 30 healthy SD rats were randomly divided into normal group， ARDS model group， and naloxone treatment group. The ARDS model was established by oleic acid 0.1 mL/kg i.v.， and levels of serum ANP and BNP， and NKA content in lung tissues were determined. The wet/dry weight ratio of the left lung was calculated， and the lung tissues were observed by light microscope and electron microscope. Results The wet/dry weight ratio of the left lung， and levels of serum ANP and BNP were significantly higher in the ARDS model group than in the normal group， and the NKA content in lung tissues was significantly lower in the ARDS model group than in the normal group（P0.01）， with evident pathological impairment of lung tissues. While compared with the ARDS model group， the pathological change in the naloxone treatment group was milder（P0.01）， with less pathological impairment of lung tissues. Conclusion Naloxone can inhibit the increase of ANP and BNP， and enhance the enzymatic activity of Na+-K+-ATP enzyme in lung tissues， thus showing a certain therapeutic effect on experimental rats with oleic acid induced acute respiratory distress syndro