安冲汤止血调经作用机理的实验研究 中医妇科学专业论文.docxVIP

成都中医药大学硕士学 成都中医药大学硕士学位论文 - - PAGE 4 - - 3.On vasoactive substance of endometrial blood vessel :Compared with normal group ,each group of ACT and Dan Zhen Ke Li group can rise up the express of ET-1,and there are significant difference(P0.05).Compared with normal group,each group of ACT and Dan Zhen Ke Li group can degrade the express of iNOS ，and the- re are significant(P0.05). CONCLUSIONS: On gen-endocrine: ACT can effect the endometrium by change the express of E2， P,PR and ER.Then may arrest and regulate menstruation. On endometrial blood vessel and vasoactive substance of endometrial blood vessel：ACT may decrease amount area of endometrial blood vessel,change the shape of endometrial blood vessel ，rise up the express of ET-1,and degrade the express of iNOS, then may effect the function of relaxation and contraction to arrest bleeding and regulate menstruation. KEY WORDS: An-Chong-Tang(ACT) Arrest bleeding Regulate menstruation; Mechanism of hemostasis 目 录 中文摘要………………………………………………………………………………1 Abstract……………………………………………………………………………… 3 目录……………………………………………………………………………………5 缩略词表………………………………………………………………………………7 引言……………………………………………………………………………………8 1.实验研究………………………………………………………………………… 9 1.1 实验研究材料材料…………………………………………………………9 1.1.1 研究对象…………………………………………………………………… 9 1.1.2 受试药物……………………………………………………………………9 1.1.3 主要试剂…………………………………………………………………… 9 1.1.4 主要仪器和设备…………………………………………………………… 10 2 实验方法………………………………………………………………………… 10 2.1 动物分组……………………………………………………………………… 10 2.2 给药方法……………………………………………………………………… 10 2.3 标本采集……………………………………………………………………… 11 2.4 检测项目及方法……………………………………………………………… 11 2.4.1 血液检测…………………………………………………………………… 11 2.4.2 病理检测…………………………………………………………………… 11 HE 染色…………………………………………………………………… 11 HE（苏木精伊红）染色………………………………………………… 11 2． HE 染色步骤………………………………………………………… 11 2.4.2．2 免疫组化……………………………………………………………… 11 .1 组织标本的采集与处理方法：……………………………………… 11 2． SP 检测方法…………………………………………………………11 分析方法………………………………………………………… 12 2.5 实验资料的统计与处理……………………………………………………… 13 3 实验结果………………………………………………………………………… 13 3.1.