脑发育不同阶段慢性铅暴露对大鼠在体海马不同时间一氧化氮含量及合酶活性影响.docVIP

脑发育不同阶段慢性铅暴露对大鼠在体海马不同时间一氧化氮含量及合酶活性影响 　　[摘要]目的：比较脑发育不同阶段低浓度慢性铅暴露对大鼠在体海马一氧化氮含量及合酶活性的影响。方法：分别在仔鼠出生21 d、42 d、63 d时测定对照组、断乳后暴露组、母体暴露组和持续暴露组仔鼠血铅含量和海马NO含量和NOS活性，并与对照组进行比较。结果：各暴露组在不同时间血铅含量均高于对照组（P0.05）。断乳后铅暴露组和持续铅暴露组NOS活性与对照组比较，差异均有显著性（P0.05），出生21 d时明显高于对照组，而42 d和63 d明显低于对照组。持续暴露组NO含量与对照组比较，差异有显著性，出生21 d时明显高于对照组，而42 d和63 d明显低于对照组。结论：脑发育任一阶段的慢性铅暴露均对海马NO含量和NOS活性有影响。与发育成熟海马相比，未成熟期海马对铅的神经毒性更为敏感，突触可塑性更易受损。 　　[关键词]慢性铅暴露；大鼠；海马；脑发育 　　[中图分类号]R995 [文献标识码]B[文章编号]1673-7210（2007）09（c）-095-02 　　 　　Effects of chronic lead exposure at brain different developmental stages and different time on NO and NOS in rat hippocampus in vivo 　　LIN Yu-shan 　　(Zhanjiang Chinese Medical College,Zhanjiang 524092,China) 　　[Abstract]Objective:To investigate the effects of chronic lead exposure at brain different developmental stages on NO and NOS in rat hippocampus in vivo.Methods: Concentration of lead in blood,concentration of NO and activity of NOS in rat hippocampus were detected at the 21,42 or 63 days after the young rat were born.Results: The concentration of lead in blood were significantly different at different time between the control and the three Pb-exposed groups.The activity of NOS and concentration of NO in rat hippocampus between lead-expoesd depart from latex group,lead-expoesd continously group and control group were significantly different.Conclusion: Chronic lead exposure in any braindevelopmental stage may have effects on the concentratioy of NO and the activity of NOS in rat hippocampus.The immature hippocampus might be higher vulnerable as compared with the mature one to lead neurotoxicity. 　　[Key words]Chronic lead exposure;Rat;Hippocampus;Brain development 　　 　　铅是环境中广泛存在的重金属污染物，具有很强的神经毒性。一氧化氮(NO)作为一种典型的逆行信使分子，具有广泛的生物学活性，在不同种群的系统发育中参与信号转导过程[1，2]，参与神经发育及海马等部位神经元突触可塑性和神经网络的构建，在学习、记忆和行为形成中起重要作用[3]。近年研究发现，基础水平的NO对神经元起保护作用，如果其生成量不足或过量，则可引起神经毒作用[4]。而一氧化氮合酶(NOS)是催化NO生物合成的关键酶，NO的生物合成主要受NOS的调节。为此本实验进行了此方面的观察，探讨并比较了脑发育不同阶段低浓度慢性铅暴露对大鼠在体海马一氧化氮含量及合酶活性及其差异，为预防和治疗铅中毒提供科学依据。 　　 　　1 材料