耐力运动对非酒精性脂肪肝小鼠肝脏NO水平、NOS活性和.PDFVIP

天津体育学院学报 ２００8 年第23 卷第6 期 Ｊｏｕｒｎａｌ ｏｆ ＴUS Ｖｏｌ．23 Ｎｏ．6 ２００8 516～518 ● 博士（生）论坛 Doctor Forum 耐力运动对非酒精性脂肪肝小鼠肝脏NO水平、NOS活性及表达 的影响 马国栋，刘艳环 摘要：目的：研究耐力运动对非酒精性脂肪肝小鼠肝脏NO水平、NOS活性及表达的影响。研究方法：雄性8周龄ApoE基因敲除小鼠24只，西方 膳食14周后，随机分为2组，非酒精性脂肪肝模型组（H,n=12，14周）,跑台运动组(E,n=12,14＋12周)。跑台运动12周，坡度为0°，跑速13m/min， 60min/time,每周5次。 在造模与训练结束后，测定肝脏总NOS、cNOS、iNOS酶活性、NO水平及eNOS和iNOSmRNA的表达，光镜观察脂肪肝病 变程度。结果：E组肝脏总NOS、iNOS酶活性和NO水平显著高于H组，cNOS未见显著性改变 肝脏实质细胞脂肪滴明显减少。结论：耐力训练在显著降低非酒精性脂肪肝肝脏脂肪滴的同时，伴随着肝脏NO含量、总NOS活性和iNOS活性 的显著增加以及eNOS和iNOSmRNA的表达上调，提示运动导致NO、NOS活性和表达的升高在非酒精性脂肪肝发病过程中可能具有重要的生理 意义。 关键词：非酒精性脂肪肝；耐力训练；一氧化氮一氧化氮合酶 中图分类号：G804.7文献标识码：A 文章编号：1005-0000（2008）06-0516-03 The Effect of Endurance Training on Level of NO, Activity of NOS and mRNA Expression of NOS in NAFLD Mice MA Guodong, LIU Yanhuan (School of PE, Shandong University of Technology, Zibo 255049, China) Abstract: Objective: To explore the effect of endurance training on level of nitric oxide, activity of nitric oxide synthase and expression of nitric oxide syn－ thase in NAFLD ApoE-knockout mice. Method: Twenty four Eight-week ApoE-knockout mice （all males）were dieted western food for 14 weeks, and ran－ domly were divided into two groups, H group and E group. E group was trained on a treadmill for 60 minutes per day, 5 days per week, 0ograde (at 13 m/ min). Every period ended, mice were sacrificed and liver was saved for measuring activities of total NOS, iNOS, cNOS, level of NO and expression of iNOS and eNOS. Results: The activities of total NOS and iNOS increased significantly in E group, while there was no significantly change of cNOS. The level of NO elevated significantly, iNOS and eNOS mRAN expression up-regulated significantly in E group. Conclusion: Endurance training decreased fat drops and increased the activity of iNOS, total NOS and the level of NO, up-regulated the mRNA expressions of iNOS and eNOS in liver. It indicated that the changes of NO