脊髓康对脊髓损伤大鼠血清脑源性神经营养因子及神经生长因子表达影响.docVIP

脊髓康对脊髓损伤大鼠血清脑源性神经营养因子及神经生长因子表达影响 　　摘要：目的 观察脊髓康对脊髓损伤大鼠血清脑源性神经营养因子（BDNF）及神经生长因子（NGF）蛋白表达的影响，探讨其改善损伤轴突再生微环境和抗脊髓损伤的作用机制。方法 90只SD大鼠采用改良Allens法建立大鼠急性脊髓损伤模型，随机抽取15只作为假手术组，其余75只造模成功后随机分为模型组、强的松组及脊髓康高、中、低剂量组。各治疗组予相应药物灌胃，假手术组和模型组予等体积生理盐水灌胃。记录期间各组大鼠活动及死亡情况，于干预后3、7、14 d处死大鼠，颈动脉采血，ELISA检测血清BDNF、NGF表达。结果 模型组大鼠脊髓损伤后均出现典型的截瘫综合征。术后3 d，BDNF表达模型组与假手术组比较差异无统计学意义（P0.05），而各治疗组均高于假手术组（P0.05，P0.01）；术后7、14 d，各治疗组和模型组均高于假手术组（P0.01），其中强的松组、脊髓康中剂量组高于模型组（P0.05，P0.01）。各治疗组和模型组NGF表达均高于假手术组，术后3、7 d各治疗组明显高于模型组；术后14 d与模型组比较，强的松组和脊髓康中、低剂量组仍维持较高水平（P0.05，P0.01）。结论 脊髓康能有效促进脊髓损伤大鼠血清BDNF、NGF表达，并在14 d内维持较高水平，从而改善轴突再生微环境，促进神经再生。 　　关键词：脊髓康；脊髓损伤；脑源性神经营养因子；神经生长因子；大鼠 　　DOI：10.3969/j.issn.1005-5304.2015.11.018 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）11-0065-04 　　Effects of Jisuikang on Expressions of BDNF and NGF in Serum of Rats with Spinal Cord Injury GUO Yang1， MA Yong1，2， DONG Wei1， PAN Ya-lan1， HUANG Gui-cheng1， YAN Rui-jie1 （1.Institute of Traumatology Orthopedics， Nanjing University of Chinese Medicine， Nanjing 210023， China；2.Affiliated Hospital of Nanjing University of Chinese Medicine， Nanjing 210029， China） 　　Abstract：Objective To clarify the effects of Jisuikang on expression levels of BDNF and NGF protein in serum of rats with spinal cord injury；To explore its probable mechanism to improve the axon regeneration microenvironment and resistance spinal cord injury. Methods Ninety SD rats were chosen to establish acute spinal cord injury model through modified Allen’s method. 15 rats were chosen randomly as sham-operation group， and the other 75 rats were randomly divided into model group， prednisone group and high-， medium-， and low-dose Jisuikang groups. All administration groups were given relevant medicine for gavage， while rats in sham-operation group and model group were given normal saline with the same volume for gavage. The activity and death condition of rats were recorded. Rats were put to death on the 3rd， 7th and 14th d of intervention for carotid artery blood collection. The exp