脊髓胶质细胞及前炎性细胞因子在慢性前列腺炎慢性盆腔疼痛综合征大鼠中作用及机制.docVIP

脊髓胶质细胞及前炎性细胞因子在慢性前列腺炎慢性盆腔疼痛综合征大鼠中作用及机制 　　【摘要】 目的 探讨脊髓星形胶质细胞和小胶质细胞以及前炎性细胞因子白细胞介素?1β(IL?1β)、白细胞介素?6(IL?6)和肿瘤坏死因子α(TNFα)在CP/CPPS发生发展中的作用及机制。方法 40只健康雄性Sprague?Dawley(SD)大鼠，随机分为正常不加任何处理的对照组(n=20)、CP/CPPS模型组(n=20)，完全福氏佐剂和3%角叉菜胶前列腺内注射造成CP/CPPS模型。通过检测机械性痛阈(PWT)来评价动物痛行为学改变；采用免疫组织化学、逆转录多聚酶链反应 (RT?PCR)等方法，检测脊髓星形胶质细胞GFAP和小胶质细胞OX?42的表达以及脊髓前炎性细胞因子IL?1β、IL?6及TNFα mRNA表达的变化，评价脊髓小胶质细胞和星形胶质细胞活化情况及脊髓前炎性细胞因子表达与疼痛行为改变的关系。结果 与对照组比较，CP/CPPS模型组大鼠建模后第11天出现PWT明显降低(P0.05)，并呈进行性下降趋势； 随着时间进程，免疫组化结果显示模型组动物脊髓小胶质细胞和星形胶质细胞依次活化，小胶质细胞活化开始于建模后第7天，星形胶质细胞活化开始于建模后第14天；模型组动物脊髓IL?1β、IL?6和TNFα mRNA表达较对照组明显增加(P0.05)。结论 在CP/CPPS大鼠中脊髓胶质细胞活化及前炎性细胞因子IL?1β、IL?6和TNF α的表达增加，可能在神经病理性疼痛的产生和维持中起着重要的作用。? 　　【关键词】 　　前列腺炎；疼痛；胶质细胞；细胞因子 　　?? 　　 　　Roles and mechanism of spinal glia and proinflammatory cytokines in rats withchronic prostatitis/chronic pelvic pain syndromes 　　 　　LIN Jian?qing, LIN Cai?zhu, LIN Xian?zhong, et al. Department of Anesthesiology,First Affiliated Hospital of Fujian Medical University,Fuzhou 350005 China 　　? 　　【Abstract】 Objective 　　To investigate the roles and mechanism of spinal glia and proinflammatory cytokinesIL?1β，IL?6 and TNF?αin the rat model ofchronic prostatitis/chronic pelvic pain syndromes Methods SD rats were randomly divided into two groups(model group and control group).The models of CP/CPPS were induced by injection of complete Freund?S adjuvant and 3％ carrageenan in prostate. The mechanical hyperalgesia (PWT) of therats was measured, and the expression levels of IL?1β, IL?6 and TNF?α in the spinal cord of neuropathic pain models and controls were measured with histochemical staining and reverse transcription polymerase chain reaction (RT?PCR). Results Theprogressivemechanical hyperalgesiahappenedafter 11 days that CP/CPPS was built. Histochemical staining showed that the GFAP and OX?42 optical density ratios ofthe spinal dorsal horn were increased with time course after vincristine injection.The levels of IL?1β,IL?6 and TNF?α were up?regulated in the spinal cord with time