肝细胞生长因子在上皮性卵巢癌血清中表达及临床意义.docVIP

肝细胞生长因子在上皮性卵巢癌血清中表达及临床意义 　　【摘要】 目的 探讨肝细胞生长因子(HGF)在上皮性卵巢癌血清中含量及其与卵巢癌早期诊断的关系。方法 血清HGF测定采用固相酶联免疫吸附试实验(ELISA)双抗体夹心法检测。结果 ①上皮性卵巢癌血清HGF各临床分期之间分别比较,P0.05,差异有显著性;②上皮性卵巢癌血清HGF各病理分级之间分别比较,P0.05,差异有显著性;③HGF和CA125对卵巢癌诊断敏感率分别为91.3%和38%,与二者联合检测相比较,P0.05,差异有显著性。结论 血清HGF值与卵巢癌临床分期和病理分级均有关,通过对HGF及血清CA-125的联合检测可以对高危人群进行早期筛查,以提高卵巢癌的早期诊断率。 　　【关键词】 上皮性卵巢癌; HGF ;CA125;酶联免疫 　　 　　The clinical value of serum hepatocyte grouth factor content in patients of epithelial ovarian carcinoma 　　 　　MA Zhi-nan.Maternal and clid care Hospital of Yangzhou City,Yangzhou 225127,China 　　 　　【Abstract】 Objective To study the expression of hepatocyte grouth factor in epithelial ovarian carcinoma and its relation with early diagnosis of ovarian carcinoma.Methods The expression of HGF in serum of epithelial ovarian carcinoma was detected with ELISA.Results ①The compare of HGF in serum in different pathological classification had significant difference(P0.05).②The compare of HGF in serum in different pathological classifycation had significant difference(P0.05);③The sensitivities of HGF and CA125 to m alignant ovarian tumor were only 91.3% and 38%,but it wouldl be up to 95% with 2 parameters at the same time.Conclusions The expression of HGF in serum is correlated with both clinical stage and pathological classification of epithelial ovarian carcinoma. The deterurination of HGF together with CA125 can obviously improve the sensitivity to ovarian cancers.The HGF in different ovarian carcinoma tissue cellular syrup serum has positive relation with the HGF in serum. 　　【Key words】 Epithelial ovarian carcinoma;Hepatocyte grouth factor;CA125;ELISA 　　 　　卵巢癌是死亡率最高的妇科恶性肿瘤。I期或II期的卵巢癌患者5年生存率为70%~90%,而且无需术后化疗;但III期或IV期的卵巢癌患者5年生存率仅为20%[1]。卵巢癌早期诊断是降低卵巢癌患者死亡率的重要措施。肿瘤标记物的监测为目前临床上所应用的一种较为理想的监测手段。目前临床已经采用血清CA125初步预测卵巢癌的预后及转归 。但是,血清CA125测定用于卵巢癌诊断的敏感度、特异度并不佳,假阳性率较高。肝细胞生长因子(HGF)是一种血管生成的强大诱导剂,其与肿瘤的浸润转移密切相关。Parrott等[2]的研究中HGF可刺激卵巢癌细胞株SKOV3和OCC1的生长,说明HGF可能参与维持卵巢肿瘤生长。HGF有望成为一种新的肿瘤标记物。上皮性卵巢癌占卵巢癌的85%~90%,在临床较常见。故本文应用酶联免疫测定了上皮性卵