肿瘤转移相关基因1与抑制基因KISS1在胰腺癌中表达及意义.docVIP

肿瘤转移相关基因1与抑制基因KISS1在胰腺癌中表达及意义 　　[摘要] 目的 研究肿瘤转移相关基因1（metastasis associated 1，MTA1）与抑制基因KISS-1在胰腺癌中的表达，探讨其与病理指标的关系。 方法 应用免疫组化Envision二步法在36例胰腺癌组织、10例癌旁胰腺组织中检测MTA1与KISS-1蛋白的表达情况。 结果 MTA1在胰腺癌组织中的阳性表达率为77.8%（28/36），显著高于癌旁胰腺组织中的表达[20.0%（2/10）]（P ＜ 0.01）；KISS-1在胰腺癌组织中的阳性表达率为38.9%（14/36），明显低于癌旁胰腺组织的90.0%（9/10）（P ＜ 0.01）。二者的表达均与患者年龄、性别及肿瘤部位无关（P ＞ 0.05），而与癌组织的分化程度、淋巴结转移及临床分期有关（P ＜ 0.05）。胰腺癌组织中MTA1与KISS-1的表达呈负相关（r = -0.533，P ＜ 0.01）。 结论 MTA1和KISS-1与胰腺癌的发生、侵袭以及转移有关，其有望成为胰腺癌诊治的重要生物学指标。 　　[关键词] 肿瘤转移相关基因1；KISS-1；胰腺癌；免疫组化 　　[中图分类号] R735.9[文献标识码] A[文章编号] 1673-7210（2012）03（c）-0070-03 　　Expression and significance of tumor metastasis associated 1 and suppressor gene KISS-1 in pancreatic cancer 　　LIU YongLIU JianshengLI JujianWANG Yan 　　Department of General Surgery, the First Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China 　　[Abstract] Objective To study the expression of tumor metastasis associated 1 (MTA1) and suppressor gene KISS-1 in the pancreatic cancer and explores its relationship with the pathological indicators. Methods The immunohistochemical Envision two-step method was used to detect the MTA1 and KISS-1 protein expression in 36 cases of pancreatic cancer tissue and 10 cases of normal paracancer tissue. Results The positive expression rate of MTA1 in pancreatic cancer was 77.8% (28/36), which was significantly higher than the 20.0% (2/10) in the normal paracancer tissue (P 0.05), but related to the differentiation degree of cancer tissue, lymph node metastasis and clinical stage (P 0.05). In the pancreatic cancer tissue, the expression of MTA1 and KISS-1 were negatively correlated with each other (r = -0.533, P 0.01). Conclusion MTA1 and KISS-1 are related to the occurrence, invasion and metastasis of pancreatic cancer and therefore are expected to become important biological indicators in the diagnosis and treatment of pancreatic cancer. 　　[Key words] MTA1; KISS-1; Pancreatic cancer; Immunohistochemistry; 　　胰腺癌是消化系统常见的恶性肿瘤，具有局部侵袭