芪参益气滴丸对糖尿病心肌病大鼠心肌纤维化影响.docVIP

芪参益气滴丸对糖尿病心肌病大鼠心肌纤维化影响 　　[摘要] 目的 探讨芪参益气滴丸对糖尿病心肌病大鼠心肌细胞纤维化的影响。 方法 将成功构建糖尿病模型的30只Wistar大鼠随机分为对照组和治疗组，每组各15只。治疗组给予芪参益气滴丸混悬液35 mg/（kg?d）灌胃，对照组给予等量生理盐水灌胃。两组分别于第3、6、9、12、15周处死大鼠，每组3只。采用TTC染色观察心肌梗死区域，采用Masson染色?^察心肌组织胶原情况。 结果 Masson 染色显示第9、12、15周治疗组大鼠心肌间质胶原纤维较对照组减少。第12、15周治疗组大鼠心肌胶原纤维面积百分比低于对照组（P0.05）。 结论 芪参益气滴丸具有抑制糖尿病心肌纤维化发生的作用，可预防糖尿病大鼠心肌纤维化。 　　[关键词] 芪参益气滴丸；糖尿病心肌病；大鼠；心肌纤维化 　　[中图分类号] R587.2；R542.2 [文献标识码] A [文章编号] 1673-9701（2017）35-0032-03 　　[Abstract] Objective To investigate the effect of Qishenyiqi dropping pills on cardiomyocyte fibrosis in diabetic cardiomyopathy rats. Methods 30 successfully constructed diabetic model Wistar rats were randomly divided into control group（n=15） and treatment group（n=15）. The treatment group was given Qishenyiqi dropping suspension 35 mg/（kg?d） gavage， and the control group was given the same amount of saline gavage. The rats in both groups were sacrificed at the third week， the sixth week， the ninth week， the twelfth week and the fifteenth week， with 3 rats in each group. TTC staining was used to observe the myocardial infarction area. Masson staining was used to observe the myocardial collagen. Results Masson staining showed that the myocardial interstitial collagen fibers in the treatment group were lower than those in the control group in the ninth week， the twelfth week and the fifteenth week. The percentage of myocardial collagen fibers area in the treatment group was lower than that in the control group in the twelfth week and the fifteenth week（P0.05）. Conclusion Qishenyiqi dropping pills can inhibit the occurrence of diabetic myocardial fibrosis and prevent myocardial fibrosis in diabetic rats. 　　[Key words] Qishenyiqi dropping pills； Diabetic cardiomyopathy； Rat； Myocardial fibrosis 　　糖尿病心肌病（Diabetic Cardiomyopathy，DCM）是糖尿病发病过程中发生的特异性心肌损伤，是糖尿病的主要并发症之一。一般认为长期的糖脂代谢紊乱影响糖尿病患者心肌组织的代谢，引起心肌微血管病变，导致大范围的心肌结构异常，从而发生左心室肥大、收缩及舒张功能障碍，最终可导致心力衰竭的形成。糖尿病心肌病的主要病理改变为心肌细胞肥大、凋亡和心脏间质纤维化。这种病理生理改变与高血压、心肌缺血等损伤因素无明显关联[1]。芪参益气滴丸是由黄芪、丹参、三七、降香经科学配伍，