血管内皮细胞生长因子基质金属蛋白酶―2和基质金属蛋白酶抑制因子―2在非小细胞肺癌中表达及意义.docVIP

血管内皮细胞生长因子基质金属蛋白酶―2和基质金属蛋白酶抑制因子―2在非小细胞肺癌中表达及意义 　　[摘要] 目的 探?非小细胞肺癌（NSCLC）组织中血管内皮细胞生长因子（VEGF）、基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶抑制因子-2（TIMP-2）的表达与肺癌侵袭和转移的关系。 方法 收集上海市普陀区中心医院2012年1月～2015年12月临床和病理确诊的56例NSCLC标本，其中25例肺鳞癌、31例肺腺癌；另取20例癌旁正常肺组织作为阴性对照，用免疫组化SP法检测标本中VEGF、MMP-2和TIMP-2的表达。 结果 VEGF、MMP-2和TIMP-2在肺癌和正常肺组织中的表达差异有统计学意义（P 0.05）。 结论 VEGF、MMP-2和TIMP-2的表达与非小细胞肺癌的侵袭和转移相关，可作为评价肺癌组织侵袭和转移能力的指标。 　　[关键词] 非小细胞肺癌；血管内皮细胞生长因子；基质金属蛋白酶-2；基质金属蛋白酶抑制因子-2；免疫组织化学；肿瘤转移 　　[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-7210（2017）03（b）-0107-04 　　Expression of vascular endothelial growth factor， matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in non small cell lung carcinoma and its clinical significance 　　YU Xuejun1 LIU Xinyan1 LI Cairong1 WANG Jinjun1 ZHAO Chuncheng2 SHENG Xia3 TAO Yujing1 　　1.Department of Geriatrics， Central Hospital of Shanghai Putuo District， Shanghai 200062， China； 2.Department of Thoracic Surgery， Central Hospital of Shanghai Putuo District， Shanghai 200062， China； 3.Department of Pathology， Central Hospital of Shanghai Putuo District， Shanghai 200062， China 　　[Abstract] Objective To study the expression of vascular endothelial growth factor （VEGF）， matrix metalloproteinase-2 （MMP-2） and tissue inhibitor of metalloproteinase-2 （TIMP-2） in non small cell lung carcinoma （NSCLC） and their relationship with invasion and metastasis of the lung cancerous tissues. Methods Fifty-six cases of human NSCLC tissues which had clinical and pathological diagnosis were collected from the Central Hospital of Shanghai Putuo District from January 2012 to December 2015. Among them， 25 cases had lung squamous cell carcinoma and 31 cases had lung adenocarcinoma. Another 20 cases of adjacent normal lung tissue were taken as a negative control. The expression of VEGF， MMP-2 and TIMP-2 were detected by SP immunohistochemical technique in all specimens. Results The expression of VEGF， MMP-2 and TIMP-2 in lung carcinoma and normal lung tissues were statistically significantly differen