论著辛伐他汀联合PMA对SHI1细胞增殖与凋亡影响.docVIP

论著辛伐他汀联合PMA对SHI1细胞增殖与凋亡影响 　　摘要： 目的探讨羟甲基戊二酸单酰辅酶A（HMG-CoA）还原酶的抑制剂辛伐他汀（SV）联合佛波酯（PMA）对SHI-1细胞增殖，分化与凋亡的影响，以及WT1/hDMP1基因的表达变化。 方法以不同浓度辛伐他汀单独或联合PMA处理SHI-1细胞，取对数生长期细胞进行实验。各组细胞分别进行MTT法观察细胞增殖能力；流式细胞测定SHI-1细胞凋亡指标AnnexinⅤ/propidium iodide变化； Real-time RT-PCR测定WT1/hDMP1基因表达变化。结果 15μmol/LSV，10μmol/LSV单独处理SHI-1细胞，随着培养时间延长，细胞抑制率增加（F=24.61，P=0.000），AnnexinV表达水平逐渐增高（F=5.69，P=0.018），WT1表达水平逐渐降低（F=12.20,P=0.008），同时伴随着hDMP1表达水平的增加（F=23.81,P=0.000），其中以15μmol/LSV联合PMA处理SHI-1细胞培养72h细胞抑制率最为明显。结论辛伐他汀体外抑制SHI-1细胞的增殖，促进SHI-1细胞的分化及凋亡，降低WT1表达，升高hDMP1表达，提示辛伐他汀具有协同治疗急性单核细胞白血病的潜能。 　　关键词：辛伐他汀；SHI-1细胞；佛波酯；增殖；凋亡；WT1；hDMP1 　　Proliferation and Apoptosis Effects of Simvastatin Combined with PMA in Human Monocytic Leukemia Cell Line SHI-1 　　JIANG Ting-xiu1, CHEN Hong1,GU Wei-ying2, QIU Guo-qiang2, WANG Zhi-lin2, HE Bai2 　　（1.Department of Hematology ,The forth affiliated hospital of Guangxi Medical University,Liuzhou 545005,Guangxi,China;2.Department of Hematology,The First People,s Hospital of Changzhou, Third Affiliated Hospital of Suzhou University,Changzhou 213003,Jiangsu,China） 　　Abstract：ObjectiveTo investigate the effect ofSimvastatin(SV) combined with Phorbol-12-myristate-13-acetate(PMA) on the proliferation ,differentiation，apoptosis and WT1/hDMP1 gene expression profiles of human monocytic leukemia cell line SHI-1. MethodsSHI-1 cells were incubated with Simvastatin and PMA solely or in combination， Cells of different groups were collected after incubation for further detection．MIT method was used to assay the growth inhibition rate and cytoflowmetry was used to detect the early stage apoptosis ratio and cell necrosis ratio．Real-time quantitative reverse transcriptase polymerase chain reaction(RT-PCR) was used to detect the WT1/hDMP1 gene expression levels. ResultsTreated with 15μmol/LSV and 10μmol/LSV in each, with the SHI-1 cells growth, the cell inhibition rates gradually increased(F=24.61,P=0.000), and AnnexinV expression levels(F=5.69,P=0.018), however the WT1 expression levels gradual