银杏叶提物对肝癌细胞生长株甲胎蛋白分泌及γGT活性影响.docVIP

银杏叶提物对肝癌细胞生长株甲胎蛋白分泌及γGT活性影响 　　【摘要】 目的 研究银杏叶提物(EGb)对肝癌细胞生长、甲胎蛋白(AFP)分泌及γ-谷氨酰转移酶(γ-GT)活性的影响。方法 以两种人肝癌细胞株SMMC-7721 细胞和Bel-7402细胞分别与EGb(17．5~105mg/d)共培养6 d,利用MTT 比色、ELISA 及细胞分化检测等方法。结果 Egb(17．5、35、70、105 mg/d)均出现细胞增殖抑制,亚细胞结构趋于正常,并且明显降低HepG2 细胞高水平分泌AFP 。EGb(35、70 mg/d)可抑制肝SMMC-7721 细胞的γ-GT 活性,升高白蛋白(ALB)含量。EGb(70、105 mg/d)亦可抑制肝癌Bel-7402细胞的γ-GT 活性,并升高ALB 含量。结论 EGb 具有抑制肝癌生长、AFP 分泌及γ-GT 活性的作用,诱导其在体外向正常肝细胞分化。? 　　【关键词】银杏叶提物;肝癌细胞；甲胎蛋白；γ-谷氨酰转移酶活性 　　 　　Effecttiontion of Hepatoma cell line growth stock、α-FP excretion and γ-glutamyl transferase activity by applying ginkgo biloba leaves extract 　　 　　SUN Xiao,PEI Xu-dong.Henan Province Nanyang Urban Central Hospital,Nanyang 473009,China 　　? 　　【Abstract】 Objective To study effecttiontion of hepatoma cell line growth stock、α-FP excretion and γ-glutamyl transferase activity by applying ginkgo biloba leaves extract(EGb).Methods To discern Vitamin D anthropo-hepatoma carcinoma cellline growth stock SMMC-7721 cell and Bel-7402 cell，to co-culture 6 days unitingEGb(17．5~105 mg/d),to utilize(Mono-nuclear cell direc cytotoxicity assay)MTT shade selection,(enzyme-linked immunosorbentassay)ELISA and cellular differentiation detecting system.Results As all to show cytostasis,subcellular structure to normalin,and obviously step down HepG2 cell homo-level secrete α-FP,using EGb(17．5、35、70、105 mg/d).Egb(35、70 mg/d)hold-back SMMC-7721 hepat-cellularγ glutamyl transferase activity,to heighten albumin contents.Eg(70、105 mg/d)hold-back Bel-7402 hepat-cellularγ glutamyl transferase activity,to heighten albumin contents.Conclusion EGb have effection which to inhibit hepatoma cell line growth stock、α-FPexcretion and γ-glutamyl transferase activity；to guide to hepat-cellular differentiation in vitro.? 　　【Key words】Ginkgo biloba leaves extract; Hepatoma carcinoma cell；α-foetoprotein(α-FP)；γ-glutamyl transferase activity 　　 　　银杏为银杏科植物(Ginkogo biloba L),其叶和果有重要药用价值,银杏提取物主要有效成分为黄酮类和萜类内酯化合物。国内外学者对银杏的化学成分、药理作用进行了广泛的研究,证实其对减轻肝细胞炎性反应/抗肝细胞纤维化有确切治疗作用。银杏叶抗