针刺对应激性胃黏膜损伤大鼠睡眠时间及纹状体中五羟色胺多巴胺含量影响.docVIP

针刺对应激性胃黏膜损伤大鼠睡眠时间及纹状体中五羟色胺多巴胺含量影响 　　摘要：目的 从脑-肠轴角度探讨针刺不同穴位对大鼠应激性胃黏膜损伤的愈合及睡眠情况的影响。方法 将雄性Wistar大鼠随机分为空白组、模型组、治胃组、安神组、联合组，每组8只。采用无水乙醇灌胃方法造成应激性胃黏膜损伤大鼠模型，治胃组穴选双侧“足三里”“中脘”，安神组穴选双侧“申脉”“照海”，联合组穴选双侧“足三里”“中脘”“申脉”“照海”，用0.22 mm×13 mm不锈钢毫针刺入5～10 mm，留针20 min。针刺干预5 d后检测热痛阈的变化。按40 mg/kg大鼠体质量腹腔注射2%戊巴比妥钠，记录注射后大鼠的睡眠持续时间。肉眼观察大鼠的胃黏膜形态，Guth法计算黏膜损伤指数（UI），高效液相色谱-电化学检测法测纹状体中5-羟色胺（5-HT）、多巴胺（DA）的含量。结果 与空白组比较，模型组热痛阈和睡眠持续时间显著下降（P0.01），UI及5-HT、DA含量显著升高（P0.05，P0.01）；与模型组比较，各治疗组热痛阈和睡眠持续时间增加（P0.05，P0.01），UI均显著降低（P0.01），治胃组和联合组中5-HT含量明显降低、治胃组DA含量显著降低（P0.01）；各治疗组间比较，联合组热痛阈及睡眠持续时间显著增加（P0.01），UI显著降低（P0.05）。结论 针刺足三里、中脘、照海、申脉可促进大鼠胃黏膜损伤的愈合，同时延长睡眠持续时间，其中四穴联合应用效果更佳，其机制可能是通过调节纹状体中5-HT及DA含量发挥作用。 　　关键词：应激性胃黏膜损伤；针刺；热痛阈；睡眠时间；胃黏膜损伤指数；5-羟色胺；多巴胺；大鼠 　　DOI：10.3969/j.issn.1005-5304.2015.05.014 　　中图分类号：R245 文献标识码：A 文章编号：1005-5304（2015）05-0048-04 　　Abstract：Objective To observe the effects of acupuncture on the sleeping quality of male Wistar rats with stress induced gastric mucosal injury from the viewpoint of the brain-intestine axis. Methods Male Wistar rats were randomized into normal control， model， ST36-CV12， BL62-KI6， and combined groups， with 8 rats in each group. The model was established by using intragastric perfusion of ethanol in the rats. Acupoints of Zusanli （ST36） and Zhongwan （CV12） on both sides were used for the ST36-CV12 group. Acupoints of Shenmai （BL62） and Zhaohai （KI6） on both sides were used for the BL62-KI6 group. All four acupoints were used for the combined group. 0.22 mm × 13 mm stainless steel filiform needles were inserted 5-10 mm deep， and were left untouched for 20 minutes before withdrawal. The acupoints were stimulated each day for five days and the paw withdraw thermal latency （PWTL） was detected after the last stimulation. 2% pentobarbital sodium was then injected （40 mg/kg）， and sleeping time of rats was detected. Gastric mucosa was observed through naked eyes. Gastric mucosal ulcer Index was determined by using the Guth method. 5-HT and DA contents were detected by using HPLC-