银杏内酯B协同地塞米松对小鼠哮喘模型气道炎症和辅助T细胞亚群影响作用研究.docVIP

银杏内酯B协同地塞米松对小鼠哮喘模型气道炎症和辅助T细胞亚群影响作用研究 　　[摘要] 目的探讨银杏内酯B协同地塞米松对支气管哮喘气道变应性炎症及辅助T细胞亚群的作用。方法40只BALB/c小鼠随机分成正常对照组、哮喘对照组、地塞米松组、银杏内酯B组和联合干预组,每组8只。收集支气管肺泡灌洗液(BALF),行白细胞(WBC)及嗜酸性粒细胞( EOS)计数;应用ELISA法测定BALF中IFN-γ和IL-4水平。结果BALF中WBC及EOS计数:3个用药组较哮喘对照组明显减少(P0.01),联合干预组减少的程度大于地塞米松组(P0.05)、银杏内酯B组(P0.01)。3个用药组小鼠BALF上清中IFN-γ和IL-4水平 与哮喘对照组比较,银杏内酯组、地塞米松组和联合干预组BALF上清中IFN-γ和IL-4水平分别增高和降低(P0.05或P0.01),而联合干预组增高和降低的程度大于地塞米松组(P0.01)、银杏内酯组(P0.05)。结论银杏内酯B能减轻小鼠哮喘模型BALF中炎性细胞浸润,并减少IL-4的水平;银杏内酯B与激素具有协同抗炎作用;同时具有更好的调节Th亚群失衡的作用。 　　[关键词] 支气管哮喘; 银杏内酯B; 气道炎症; T辅助细胞亚群 　　[中图分类号] R969 [文献标识码] A [文章编号] 1673-9701(2009)23-25-02 　　 　　Effects of Ginkgolide B Coordinated with Dexamethasone on Allergic Airway Inflammation and T Helper Cell Subsets in Asthmatic Rats 　　LV Jinquan ZHANG Xiaoming LV Jianping XIN Yue TANG Yan 　　Pediatrics Department,the Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China 　　 　　[Abstract]ObjectiveTo investigate the effects of Ginkgolide B(GB)coordinatedwith Dexamethasone(DXM)on allergic airway inflammation and T helper cell subsets in asthmatic rats. Methods Forty BALB/c rats were randomly divided into 5 groups equally:the normal group,the asthma model group,the DXM group,the GB group and the DXM+GB group. The WBC count and eosinophil(EOS)count in bronchoalveolar lavage fluid(BALF) were estimated and ELISA was used to determine the concentrations of IFN-γ and IL-4 in BALF. ResultsCompared with the asthma model group,EOS and WBC count and IL-4 level in BALF were significantly lower in the three treated groups(P0.01),particularly in the DXM+GB group,showing a significant difference as compared with the other two groups(P0.05 or P0.01),whereas IFN-γ level was increased. And in the DXM+GB group,they showed a significant difference as compared with the other two groups(P0.05 or P0.01). ConclusionGingkgolide B has an inhibitory effect synergistic with Dexamethasone in airway inflammation,and might partly reverse T helper cell subsets in rats with asthma. 　　[Key Words]Asth