酸碱性氧化电位水替代硫酸洗液实验研究.docVIP

酸碱性氧化电位水替代硫酸洗液实验研究 　　[摘要] 目的：探讨酸性氧化电位水(EOW)和碱性氧化电位水(AOW)替代硫酸-重铬酸钾液清洗玻璃器皿，用于细胞培养、生物材料、生化检测等实验的可行性。方法：取玻璃培养瓶、试管和吸管各100个（只），洗涤灵洗刷、自来水冲净，烤干后分3组浸入各浸泡液中。A组：硫酸-重铬酸钾洗液；B组：EOW；C组：AOW。浸泡24 h后取出，自来水冲洗10遍，双蒸水2遍、三蒸水1遍，烤干，高压蒸汽灭菌后接种RSC-364大鼠滑膜细胞，分别进行细胞生长速度、贴壁率、存活率和活细胞形态比较。结果：细胞生长速度测定：1～3 d为对数长生期，平均倍增时间A组为26 h，B组34.7 h，C组25.8 h；4～5 d为平台期，细胞生长减慢；第6天细胞停止生长，增殖曲线迅速下降。细胞贴壁率：A组74%，B组65%，C组70%（P0.05）。细胞存活率：A组93.5%，B组94.1%，C组94.6%（P0.05）。三组细胞形态变化不大，接种后第1天，95%的细胞由圆形逐渐贴壁成长梭形；第5天细胞长满瓶壁，生长减慢。结论：A、B、C三组细胞生长速度接近，酸、碱性氧化电位水均可替代硫酸洗液清洗玻璃器皿，用于细胞培养。 　　[关键词] 硫酸洗液；酸性氧化电位水；碱性氧化电位水 　　[中图分类号]Q813.1[文献标识码]A [文章编号]1673-7210（2007）08（a）-035-02 　　 　　The experimental study of electrolyzed oxidizing water and alkalized oxidizing water in replacing sulfuric acid solution 　　ZUO Xiang-hua, CHEN Jian-kui, JIN Xin, HUANG Yuan, LI Yu-rong, ZHENG Na-xin,ZHU Xiao-hua 　　(Department of Medical Laboratory, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071，China) 　　[Abstract] Objective: To study the effect of electrolyzed oxidizing water and alkalized oxidizing water replacing sulfuric acid solution on the cleaning of cell culture glassware. Methods: The glass culture bottles, test tubes and suction tubes were randomly divided into three groups. Group A immersed in sulfuric acid, group B in electrolyzed oxidizing water (EOW), group C in alkalized oxidizing water (AOW). 24 hours later, they were dealt with asusural, and then cultivated RSC-364 cells. The cell growth rate, attachment efficiency, survival rate and cell shape were compared respectively. Results: Cell doubling time was 26 hours in group A, 34.7 hours in group B, 25.8 hours in group C. Attachment efficiency of group A was 74%, group B was 65% and group C was 70%. Cell survival rate of group A was 93.5%, group B was 94.1% and group C was 94.6%. The changes of cell shape were not obvious. Conclusion: The cell growth rates of three groups are similar. The application of electrolyzed oxidizing water and alkalized oxidizing water has su