野生大豆育成品种与其亲本间SSR聚类分析.docVIP

野生大豆育成品种与其亲本间SSR聚类分析 　　摘要：采用分布于大豆（Glycine max）全基因组的90对SSR引物，对来自全国在生产上大面积推广应用的具有野生大豆（Glycine soja）血缘的10个大豆育成品种及其17个亲本材料进行聚类和遗传距离分析。结果表明，当遗传相似系?滴?0.67时，所有供试材料被分成三类，即育成的大豆品种与其栽培大豆亲本聚为一类，野生大豆亲本在三类中均有分布；野生大豆亲本与栽培大豆亲本间的遗传距离0.177 6野生大豆亲本与育成大豆品种间的遗传距离0.149 0栽培大豆亲本与育成大豆品种间的遗传距离0.092 9。表明野生大豆与栽培大豆间有较大的遗传差异性，应用遗传基础不同及遗传差异性较大的野生大豆与栽培大豆之间进行杂交选择，进而拓宽大豆的遗传基础、丰富大豆遗传多样性是有效和可行的。 　　关键词：野生大豆（Glycine soja）；育成品种；聚类分析；遗传距离；SSR 　　中图分类号：S565.1 文献标识码：A 文章编号：0439-8114（2017）12-2215-04 　　DOI：10.14088/j.cnki.issn0439-8114.2017.12.005 　　SSR Cluster Analysis between Cultivars and Their Parents in Wild Soybean 　　L？?B Zhu-zhang1，WANG Wen-zhe1，LIANG Qing1，QIU Li-juan2 　　（1. Rizhao Polytechnic College， Rizhao 276826， Shandong， China； 2. The Key Open Laboratory of Crop Germplasm and Biotechnology of the Ministry of Agriculture/the Major Project of Crop Gene Resources and Genetic Improvement of China/Crops Research Institute， Chinese Academy of Agricultural Sciences， Beijing 100081， China） 　　Abstract： 90 pairs of SSR primer distributed in soybean genome were used for clustering and genetic distance analysis of 10 improved soybean varieties with wild soybean blood and their 17 parental materials. Results showed that when the genetic similarity coefficient was 0.67，all tested materials were divided into three groups， namely improved soybean varieties and cultivated soybean parents together as a class，wild soybean parents were distributed in all three categories. The genetic distance of 0.177 6 between wild soybean parents and cultivated soybean parentsthe genetic distance of 0.149 0 between wild soybean parents and improved soybean varieties the genetic distance of 0.092 9 between cultivated soybean parents and improved soybean varieties.It indicated that there were large genetic differences between wild and cultivated soybean， and it was feasible and effective to expand soybean genetic basis and enrich soybean genetic diversity by hybridization and selection between wild soybean and cultivated