醋制南五味子对2型糖尿病大鼠脂代谢影响.docVIP

醋制南五味子对2型糖尿病大鼠脂代谢影响 　　摘要：目的 观察南五味子醋制前后饮片对2型糖尿病（T2DM）大鼠脂代谢的影响，探讨其相关机制。方法 以高脂饲料加链尿佐菌素联合诱导制作T2DM大鼠模型。实验大鼠随机分为空白组、模型组、南五味子大剂量组、南五味子小剂量组、醋制南五味子大剂量组、醋制南五味子小剂量组，各给药组给予相应药物灌胃，连续30 d。测定空腹胰岛素（FINS）、游离脂肪酸（FFA）、总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）的含量及肝脏组织苹果酸脱氢酶（MDH）、总蛋白含量，HE染色观察大鼠肝脏和胰腺组织形态学变化。结果 与模型组比较，南五味子和醋制南五味子各剂量组对FBG、FINS改善作用不明显，但能不同程度地升高HDL-C、MDH，降低FFA、LDL-C、TC、TG，其中醋制南五味子调节T2DM大鼠糖脂代谢效果显著。结论 醋制能增强南五味子对T2DM大鼠脂代谢的调控作用。 　　关键词：南五味子；醋制；2型糖尿病；脂代谢；大鼠 　　DOI：10.3969/j.issn.1005-5304.2017.05.016 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）05-0067-04 　　Effects of Vinegar Fructus Schisandrae Sphenantherae on Lipid Metabolism in Rats with Type 2 Diabetes DENG Chong， JIANG Yi， ZHANG Hua-wei， LIU Ji-ping， CHAI Si-jia， HOU Jian-ping （Shaanxi University of Chinese Medicine， Xianyang 712046， China） 　　Abstract： Objective To investigate the effects of Schisandrae Sphenantherae Fructus and processed with vinegar on lipid metabolism of rats with type 2 diabetes mellitus （T2DM）. Methods The rat model of T2DM was induced by high fat diet plus STZ. The rats were randomly divided into blank group， model group， Schisandrae Sphenantherae Fructus high-dose group and low-dose group， and vinegar Schisandrae Sphenantherae Fructus high-dose group and low- dose group. The rats in each group were fed with the corresponding medicine for gavage for 30 d. FINS， FFA， TC， TG， HDL-C， LDL-C， and MDH， total protein content of liver tissue were detected. HE staining was used to observe the histomorphological changes of liver and pancreas in rats. Results Compared with the model group， Schisandrae Sphenantherae Fructus groups and vinegar Schisandrae Sphenantherae Fructus groups did not show obvious effects on improving FBG and FINS， but it could raise varying degrees of HDL-C and MDH， and reduce FFA， LDL-C， TC， and TG， among which vinegar Schisandrae Sphenantherae Fructus could significantly regulate metabolism in T2DM rats. Conclusion Vinegar Schisandrae Sphenantherae Fructus can enhance the li