高压氧及Notch信号阻断剂对大鼠颅脑损伤后海马区神经干细胞增殖分化影响.docVIP

高压氧及Notch信号阻断剂对大鼠颅脑损伤后海马区神经干细胞增殖分化影响 　　[摘要]目的 观察高压氧及Notch信号阻断剂（DAPT）对大鼠颅脑损伤后内源性神经干细胞增殖分化的影响。方法 100只成年健康Wistar大鼠随机平均分为假手术组、颅脑损伤组、DAPT{N-[N-（3，5-Difluorophenacetyl-L-alanyl）]-Sphenylglycinet-butylester}组、高压氧组及二甲基亚砜（DMSO）组，应用改良的自由落体方法制备脑损伤模型，用免疫荧光双标记染色法观察伤后7、14、21 d及28 d大鼠海马区神经干细胞增殖分化。结果 颅脑损伤组、DAPT和高压氧组海马区BrdU/nestin双标阳性细胞数，在伤后7 d为（14.2±1.3）、（11.4±1.7）个和（22.3±1.5）个，在伤后14 d为（9.6±0.7）、（6.9±2.1）个和（13.8±1.4）个，在伤后7 d较伤后14 d明显增多（P0.05）；与颅脑损伤组比较，DAPT组在伤后7 d及14 d双标阳性的细胞数明显减少（P0.05）；高压氧组在伤后7 d及14 d双标阳性的细胞数明显增多（P0.05）。结论 高压氧能促进内源性神经干细胞增殖分化，Notch信号阻断剂抑制了内源性神经干细胞增殖分化。 　　[关键词]高压氧；颅脑损伤；神经干细胞；Notch通路；增殖分化 　　[中图分类号] R651.2 [文献标识码] A [文章编号] 1674-4721（2017）10（a）-0007-04 　　[Abstract]Objective To observe the effects of hyperbaric oxygen therapy and DAPT on the proliferation and differentiation of endogenous neural stem cells after traumatic brain injury.Methods 100 adult Wistar rats were randomly divided into sham-operation group，traumatic brain injury group，DAPT group，hyperbaric oxygen therapy group and DMSO group.Animal models of craniocerebral trauma were made using the improved free-fall method in the rats.The proliferation and differentiation of neural stem cells of the DG brain tissue was detected by BrdU labeling fluorescence immunocytochemistry at the 7，14，21 d and 28 d after injury.Results The BrdU and nestin positive cells of the traumatic brain injury group，DAPT group and hyperbaric oxygen therapy group began to increase from the 7 d（14.2±1.3，11.4±1.7，22.3±1.5） to the 14 d after injury （9.6±0.7，6.9±2.1，13.8±1.4）.Compared with the 14 d，the BrdU and nestin positive cells significantly increased 7 d after injury （P0.05）.Compared with the traumatic brain injury group，the BrdU and nestin positive cells significantly decreased in the DAPT group 7 d and 14 d after injury （P0.05）.Compared with the traumatic brain injury group，the BrdU and nestin positive cells significantly increased in the hyperbaric oxygen therapy group 7 d and 14 d after injury （P0.05）.Conclusion Hyperbaric oxy