嗜盐放线菌YL产纤维素酶活性研究--毕业论文设计.docVIP

PAGE 嗜盐放线菌YL产纤维素酶活性研究 系 别： 生 命 科 学 系 学科专业： 食品科学与工程 姓 名： 指导教师： 2011年5月 嗜盐放线菌YL产纤维素酶活性研究 摘要：本课题对运城盐湖嗜盐放线菌YL产纤维素酶酶学性质进行了研究。DNS法对菌株YL的酶活进行测定。研究表明，该纤维素酶在50℃-90℃范围内酶活性较高，最适反应温度为60℃，说明该酶具有耐热的特性；酶促反应的最适pH是8.0，且在pH 7.0-9.0维持相对较高的酶活性，表明该纤维素酶耐碱性较好；该酶在盐度5%-17%范围内相对酶活达70%以上，最适反应盐浓度为10%，表明该纤维素酶是耐盐酶，而不是嗜盐酶；金属离子Mg2+、Zn2+对酶促反应有促进作用，Mn2+、Ca2+对该酶促反应部分抑制，而Cu2+、Fe2+对该酶促反应完全抑制，说明该酶活性的发挥受金属离子的影响较大；在特异性酶抑制剂DPEC、PMSF的作用下，相对酶活力较高，说明该纤维素酶酶活中心可能不含组氨酸和丝氨酸；同时该酶在金属螯合剂EDTA-Na的作用下，酶活力偏低，表明该酶活性的发挥依赖某种金属离子的参与。本研究对于进一步研究和应用纤维素酶具有深远意义。 关键词：嗜盐放线菌；纤维素酶；酶学性质 The activity research of cellulase producted by halophilic actinomycete YL Yuan Xueyun (Life Science College, Yuncheng University, Yuncheng Shanxi，044000) Abstract: The research aimed to study the characteristics of cellulase, produced by halophilic actinomycete YL from salt lake in Yuncheng. Acquire the cellulose enzyme activities by DNS method. The research showed as follows: the cellulase had a relatively high enzyme activity between 50℃ and 90℃, and the optimal reaction temperature is 60℃, this stated that the cellulase is thermotolerant; the optimal reaction pH value is 8.0, and the cellulase maintained a relatively activity between pH 7.0 and pH9.0, this indicated that the cellulase is alkali-resisting; the enzyme keeped the characteristics of over 70% in salinity between 5% and 17%, and the optimal reaction salinity is 10%, this revealed that the cellulase was a salt-tolerant enzyme, not a halophilic enzyme; Mg2+ and Zn2+ could activate its enzyme activity, Mn2+ and Ca2+ could partly reduce enzyme activity, while Cu2+ and Fe2+ could completely reduce the enzyme activity, this stated that metallic ions had a great influence on the cellulase; Under specific enzyme inhibitors DPEC and PMSF, the enzyme activity is relatively high, indicated that the activity center didn’t include serine and histidine; Under the EDTA-Na the enzyme activity i