下咽鳞状细胞癌患者外周血T淋巴细胞亚群与NK细胞活性研究.docVIP

下咽鳞状细胞癌患者外周血T淋巴细胞亚群与NK细胞活性研究 　　【摘要】 目的：分析下咽鳞状细胞癌患者外周血T淋巴细胞亚群和NK细胞活性情况，旨在为相关研究工作提供参考资料。方法：择取2011年6月-2016年5月本院收治的95例下咽鳞状细胞癌病患为病例组，另取此期间内来本院接受健康检查的72例志愿者为正常组。使用流式细胞仪对病患开展相关检查，分析病例组与正常组的淋巴细胞亚群、NK活性情况以及不同临床分期的下咽癌病患外周血T淋巴细胞亚群以及NK细胞活性之间的关系。结果：与正常组相比，病例组的NK细胞、总B淋巴细胞、总 　　T淋巴细胞、辅助/抑制T细胞、辅助T细胞偏低（P0.05）。Ⅰ～Ⅱ期病患辅助T淋巴细胞比Ⅲ～Ⅳ期者略高（P0.05）。Ⅰ～Ⅱ期病患抑制T淋巴细胞值比Ⅲ～Ⅳ期者低（P0.05）。Ⅰ～Ⅱ期病患外周血NK细胞数比Ⅲ～Ⅳ期者略高（P0.05）。结论：下咽鳞状细胞癌病患的免疫功能异常，且随着疾病的进展，细胞免疫和体液免疫均有所降低，证实免疫功能紊乱在疾病进展中发挥了一定作用。经流式细胞设备对外周静脉血淋巴细胞亚群以及NK细胞活性情况检测，可实现动态化检测癌症病患某阶段细胞免疫功能变化详情，此法方便简单，在指导治疗方案制定以及监测疾病预后方面，均体现出了一定效果，值得?M一步推广。 　　【关键词】 下咽鳞状细胞癌； 外周血； T淋巴细胞亚群； NK细胞 　　Research on T Lymphocyte Subsets and NK Cells Activity in Peripheral Blood of Hypopharyngeal Squamous Cell Carcinoma/JIANG Yuanqin，CHEN Aimin，HUANG Xuge，et al.//Medical Innovation of China，2017，14（35）：063-066 　　【Abstract】 Objective：To analyze the peripheral blood T lymphocyte subsets and NK cell activity in patients with hypopharyngeal squamous cell carcinoma，and to provide reference for related research work.Method：From June 2011 to May 2016 in our hospital 95 cases of hypopharyngeal squamous cell cancer were selected the case group，the other from this period came to our hospital for health examination of 72 volunteers were selected as the normal group.Using the relevant examination by flow cytometry，the relationship between the case group and the normal group of lymphocyte subsets and the activity of NK and different clinical staging of hypopharyngeal cancer patients peripheral blood T lymphocyte subsets and NK cell activity were analyzed.Result：Compared with the normal group，NK cells，total B lymphocyte，total T lymphocytes，helper/suppressor T cells，and helper T cells were lower in the case group（P0.05）.Helper T lymphocytes of Ⅰ-Ⅱ stage was slightly higher than that of Ⅲ-Ⅳ stage（P0.05）.The value of T lymphocyte suppression of Ⅰ-Ⅱ stage was lower than that of Ⅲ-Ⅳ stage（P0.05）.The number of NK cells in peripheral blood of patients of Ⅰ-Ⅱstage was slightly higher than that of Ⅲ-Ⅳ sta