鼠曲草醇提物改善慢性阻塞性肺疾病模型大鼠气道炎症实验研究.docVIP

鼠曲草醇提物改善慢性阻塞性肺疾病模型大鼠气道炎症实验研究 　　【摘要】目的：研究鼠曲草醇提物对慢性阻塞性肺疾病模型大鼠气道炎症的影响。方法：采用气管内滴注脂多糖（LPS）加烟熏法建立COPD大鼠模型，造模后第15 日开始药物干预，实验结束采用细胞计数法，计算肺泡灌洗液（BALF）细胞中巨噬细胞、中性粒细胞及淋巴细胞的比例；采用ELISA法测定BALF中IL-8和TNF-α浓度；同时采用RT-PCR法检测各组大鼠肺组织中IL-8、TNF-α的表达水平。结果：与模型组相比，鼠曲草醇提物组明显减少大鼠BALF中白细胞、巨噬细胞和中性粒细胞百分比（P0.05），而淋巴细胞百分比无显著性差异。与模型组相比，鼠曲草醇提物组明显降低大鼠BALF中IL-8、TNF-α浓度（P0.05），同时鼠曲草醇提物组能够显著下调肺组织中IL-8、TNF-α mRNA的表达水平（P0.05）。结论：鼠曲草醇提组可降低COPD大鼠BALF中白细胞、巨噬细胞和中性粒细胞百分比，降低COPD大鼠BALF中细胞因子IL-8和TNF-α的浓度及mRNA表达水平。 　　【关键词】 鼠曲草醇提物；慢性阻塞性肺疾病；IL-8；TNF-α 　　【中图分类号】R-332 【文献标志码】 A 【文章编号】1007-8517（2015）19-0005-03 　　Abstract：Objective To study the effect of ethanol extract of Gnaphalium affine on inflammation of chronic obstructive pulmonary disease （COPD） model rats. Methods The COPD model was established by smoking and infusing lipopolysaccharide （LPS）. After 15 days， the rats were given the drugs by orally taking once a day. Ratio of macrophage， neutrophile granulocyte and lymphocytes in BALF cells were evaluated by cytometry. The level of IL-8， TNF-α in BALF were measured by ELISA. Futhermore， the level of IL-8， TNF-α in lung tissue were analysed by RT-PCR. Results Compared with the model group， ethanol extract of Gnaphalium affine obviously reduced the ratio of leukocyte， macrophage and neutrophile granulocyte in BALF cells （P0.05）， but the ratio of lymphocytes had no significant difference. Compared with the model group， ethanol extract of Gnaphalium affine obviously decreased the level of IL-8， TNF-α in BALF， as well as the level of IL-8， TNF-α in lung tissue （P0.05）. Conclusion Ethanol extract of Gnaphalium affine has protective effect on lung tissue of COPD rats， and can adjust the level of IL-8， TNF-α， which may be one of its mechanisms of intervening occurrence and development of COPD. 　　Keywords：COPD；Gnaphalium affine；IL-8；TNF-α 　　慢性阻塞性肺疾病 （chronic obstructive pulmonary disease，COPD） 是一种以不完全可逆的气流受限为特征的疾病，气流受限呈进行性发展。COPD是呼吸系统常见病，多发病， 常与慢性支气管炎和肺气肿密切相关。流行病学调查发现，其死亡率居全球所有死亡原因的第4位[1]。COPD已成为当今严重的全球性公共卫生问题并成为国内外呼