《窑湾甜油面菌块中细菌的筛选鉴定》毕业学术论文.docVIP

PAGE lw lw 图书分类号： 密 级： 毕业设计(论文) 窑湾甜油面菌块中细菌的筛选鉴定 THE SCREENING AND IDENTIFICATION OF BACTERIA IN THE FERMENTED FLOUR OF YAO WAN SWEET OIL 学生姓名 xxx 学院名称 食品（生物）工程学院 专业名称 生物工程 指导教师 xxx 2012年 5月 20日 lw 摘要 窑湾甜油是极鲜美的调味品，味道极其鲜美。窑湾甜油还是一种保健食品，具有很好的保健作用。证实常年食用甜油的人高寿、血压正常，很少有脱发、早白发现象。甜油入肾经，可滋阴养肾、增强人体免疫力，有健胃、美容的作用。但窑湾甜油目前主要是以传统天然发酵的方法来生产，生产周期较长，易受外部环境的影响，存在多种不确定因素。本实验通过筛选窑湾甜油面菌块中的细菌来分析其中的细菌菌群，确定各种细菌的数量，并对筛选到的细菌进行生理生化鉴定，包括淀粉水解实验，甲基红实验，糖发酵实验，吲哚实验，明胶液化实验，尿素酶实验来鉴定，然后再用分子生物学鉴定来确定其分类地位。为进一步明确细菌在窑湾甜油的发酵中所起作用打下基础。 关键词 细菌；窑湾甜油；生理生化；鉴定 Abstract Yaowan sweet oil is a very tasty condiment,tastes very delicious.It has good health effects. It confirms people who eat sweet oil for many years are high, normal blood pressure, there is little hair loss, early white hair phenomenon. Sweet oil into the kidney, nourishing yin nourishing, can enhance human immunity, make the stomach health, the role of beauty.But it is currently manufactured by traditional natural fermentation method and a longer production cycle is needed which cause it is vulnerable to the environment and many uncertain factors exist. In the present study, bacteria in Yaowan sweet oil block were screened to determine the number of a variety of bacteria and the taxonomic status of bacteria was determined by physiological, biochemical and molecular biological methods including amylase hydrolysis, methyl red experiments sugar fermentation test, indole test, gelatin liquefaction experiments, urease test, amylase hydrolysis experiments to identify and then and then the identification of molecular biology to determine its taxonomic status. This study erects a foundation for further clarifying the role of bacteria in the fermentation of Yaowan sweet oil. Keywords bacteria Yaowan sweet oil physiological and biochemical identification