中国芒叶绿体DNAtrnL-F序列变异及遗传结构分析-中国科技论文在线.PDF

中国科技论文在线 中国芒叶绿体DNA trnL-F 序列变异及遗传 结构分析# 严海东，张新全，付晨，黄琳凯，聂刚** 5 （四川农业大学动物科技学院草业科学系，四川雅安 625014 ） 摘要：中国芒 （Miscanthus sinensis）是重要的能源植物之一，同时在干扰的生境中也是 一种重要的先锋植物。为了明确中国西南区中国芒群体的叶绿体 DNA 序列多样性和遗传结 构，本试验对14 个中国芒群体共75 个个体的叶绿体基因片段trnL-F 进行序列的测定与分 10 析，结果表明：75 个个体共检测到 5 个单倍型，13 个多态位点，占位点总数的4.43%，其 中2 处碱基替换，6 处插入/缺失，5 处同时存在碱基替换与插入/缺失。14 个群体的总遗传 多样性较低（h=0.507，π=0.00794），可能因为叶绿体基因反映有效群体较小造成群体的 遗传漂变，使得群体多样性降低。5 个单倍型的网络中介图结果表明单倍型H1 和H2 可能为 古老的单倍型。 15 关键词：中国芒；叶绿体DNA；序列变异；遗传结构；遗传多样性 中图分类号：S564 Chloroplast DNA trnL-F variation and genetic structure of Miscanthus sinensis 20 YAN Haidong, ZHANG Xinquan, FU Chen, HUANG Linkai, Nie Gang (Department of grassland science, Animal Science and Technology College, Sichuan Agricultural University, Yaan, Sichuan, 625014, China) Abstract: Miscanthus sinensis is one of dominant perennial grasses in East Asia, and is an important pioneer species in disturbed habitats. In order to obtain basic information about the 25 genetic diversity and structure of Miscanthus sinensis in south of China, where is rich in Miscanthus, our study detected the sequence variation in 75 individuals from 14 populations by using chloroplast DNA trnL-F. The results showed that 5 haplotypes and 13 polymorphic sites (4.43%), including 2 substitutions, 6 indels, and 5 sites existing substitutions and indels, were detected through splicing these two gene segments. The low genetic diversity of population was 30 founded (h=0.507, π=0.00794), which may be affected by genetic drift caused by small