超高效液相色谱法测定饮用水中壬基酚辛基酚.PDF

超高效液相色谱法测定饮用水中壬基酚、辛基酚 张珏 奚晔 詹铭* 浦东新区疾病预防控制中心,上海 200136 摘要：目的 建立了饮用水中壬基酚、辛基酚的 UPLC 分析方法。方法 样品经二氯甲烷 液液萃取、浓缩定容后经 UPLC 分析。结果 本方法线性范围在 1-50 μg /L ，方法的检测下 限在 0.005-0.020 μg /L ，加标回收率为 72%~115%，相对标准偏差2.7~7.0% 。结论 本方法 灵敏度高、操作简单、线性范围宽等优点，适用于饮用水中壬基酚、辛基酚的检测 关键词：壬基酚；辛基酚；饮用水；超高效液相色谱 Determination of nonylphenol and octylphenol in drinking-water using uplc ZHANG Jue,XI Ye, ZHAN Ming* （Pudong Centrefor Disease Control and Previention, Shanghai 200136, China Corresponding author:ZHAN Ming ，E-mail: mzhan@pdcdc.sh.cn ） Abstract: Objective The method was established to determine Nonylphenol and Octylphenol in drinking-water by uplc . Methods The water sample was extracted with dichloromethane.After concentrated,the target analytes were performed chromatographic separation and detection with uplc . Results The linear ranges of the calibration curves for Nonylphenol and Octylphenol were 1-50 μg /L. The method detection limit (MDL) for Nonylphenol and Octylphenol were 0.005- 0.020 μg /L. The average recoveries of Nonylphenol and Octylphenol in drinking-water ranged from 72% to 115%, and the relative standard deviations were 2.7~7.0%. Conclusion The method is applicable to the determination of Nonylphenol and Octylphenol in drinking water with the advantages of simple,low detection limit and good reproducibility. [Key words] Nonylphenol;Octylphenol;Drinkring-water; UPLC 环境内分泌干扰物（EDCs ）也称为环境激素，是指存在于环境中对人类和动物体内的 激素产生影响，干扰机体正常内分泌物质的合成与代谢、激活或抑制内分泌系统功能的外 源性化学物质[1]。包括烷基酚类、邻苯二甲酸酯类、农药类、重金属等。烷基酚作为一种 合成的非离子表面活性剂的原料，由于高效廉价而被广泛应用。烷基酚中以壬基酚、辛基 酚为代表，具有较大的毒性，且不易降解。目前，文献报道的水中烷基酚的检测方法主要 有衍生-气相色谱/质谱(GC/MS)法、液相色谱-紫外检测器（LC-UV ）法、液相色谱-荧光检 测器（LC-FL ）法、高效液相色谱- 串联质谱（HPLC-MS/MS ）等[2-6]。其中LC-UV 、LC- FL 法难以排除基质干扰的影响，使用受到限制。富集技术基本都集中在液液萃取和固相萃 取技术的应用。由于烷基酚类物质的污染较普遍，试剂、耗材中均能检出，采用固相萃取 技术的本底污染较难控制。本研究建立液液萃取-超高效液相色谱-荧光法检测水中壬基酚、 辛基酚，进行